Adh1 is transcriptionally active but its translational product is reduced in a rad mutant of rice (Oryza sativa L), which is vulnerable to submergence stress
Adh1 is transcriptionally active but its translational product is reduced in a rad mutant of rice (Oryza sativa L), which is vulnerable to submergence stress
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DOI：
10.1007/s001220051010
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发表时间：
1998-12-01
影响因子：
5.4
通讯作者：
Uchimiya, H
中科院分区：
文献类型：
--
作者：
Matsumura, H;Takano, T;Uchimiya, H
对 ADH 活性 (rad) 降低的单一隐性水稻 (Oryza sativa L.) 突变体的酶活性和乙醇脱氢酶 (ADH) 转录本进行了分析。先前已证明，所有 ADH 同工酶均低于需氧生长的 rad 幼苗的检测水平（Matsumura 等人，1995）。本研究表明，ADH 活性在 rad 幼苗中是厌氧诱导的，并且主要位于根部，主要位于根冠区域。在浸水的 rad 幼苗中观察到 ADH 同工酶的最低条带（ADH2 同二聚体）。尽管野生型中丰富的两种同工酶（ADH1 同二聚体和异二聚体）在该突变体中丢失，但 Adh1 mRNA 在通气或浸水的 rad 植物中正常产生。蛋白质分析表明，rad 幼苗中 ADH 蛋白的量减少。最有可能的是，Adh1 mRNA 没有翻译，或者其产物在 rad 植物中不稳定。通过芽的生长和绿化来评价沉水幼苗的活力。我们的结果表明，rad 幼苗比野生型幼苗更容易受到长期淹没胁迫的影响。
Enzyme activities and transcripts of alcohol dehydrogenase (ADH) were analyzed in a single recessive rice (Oryza sativa L.) mutant with reduced ADH activity (rad). It has been previously demonstrated that all ADH isozymes are below the level of detection in aerobically grown rad seedlings (Matsumura et al. 1995). The present study showed that ADH activity was anaerobically inducible in rad seedlings and predominantly localized in the roots, mainly in the root-cap region. The lowest band (ADH2 homodimer) of the ADH isozymes was observed in submerged rad seedlings. Although two isozymes (the ADH1 homodimer and heterodimer), abundant in the wild-type, were lost in this mutant, Adh1 mRNA was produced normally in aerated or submerged rad plants. Protein analysis showed that the amount of ADH protein was reduced in rad seedlings. Most likely, Adh1 mRNA was not translated or else its product was unstable in rad plants. The viability of submerged rad seedlings was evaluated by the growth and greening of the shoots. Our results showed that rad seedlings were more vulnerable to a long-term submergence stress than wild-type seedlings.