Expression of tumor necrosis factor-α-related apoptosis-inducing ligand and its receptors in rat testis during development
Expression of tumor necrosis factor-α-related apoptosis-inducing ligand and its receptors in rat testis during development
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DOI：
10.1095/biolreprod66.6.1707
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发表时间：
2002-06-01
影响因子：
3.6
通讯作者：
Benahmed, M
中科院分区：
文献类型：
--
作者：
Grataroli, R;Vindrieux, D;Benahmed, M
肿瘤坏死因子-α相关凋亡诱导配体（TRAIL）是细胞因子的肿瘤坏死因子-α家族的成员，已知其在与其含有死亡结构域的受体DR 4/TRAIL-R1和DR 5/TRAIL-R2结合后诱导凋亡。另外两种TRAIL受体DcR 1/TRAIL-R3和DcR 2/TRAIL-R4缺乏功能性死亡结构域，并作为TRAIL的诱饵受体。在这项研究中，TRAIL及其受体的存在进行了研究，在大鼠睾丸发育过程中。TRAIL及其受体免疫定位于不同的睾丸细胞类型。在大鼠睾丸组织中也发现了TRAIL及其受体的蛋白和mRNA。我们的免疫组织化学研究表明，TRAIL，DR 5/TRAIL-R2，和DcR 2-TRAIL-R4检测Leydig细胞，而配体和所有受体定位于生殖细胞。从胎儿期到成年期，TRAIL在生殖细胞中被永久免疫检测到，而其受体仅在减数分裂后的生殖细胞中被免疫定位。肿瘤坏死因子相关凋亡诱导配体及其受体mRNA的表达与肿瘤坏死因子相关凋亡诱导配体及其受体蛋白的免疫检测结果一致。事实上，从胎儿期到成年期的大鼠睾丸中也发现了肿瘤坏死因子相关凋亡配体mRNA。死亡受体DR 4/TRAIL-R1和DR 5/TRAIL-R2的mRNA在围产期检测到微弱的表达，从青春期到成年期逐渐增加。诱饵受体DcR 1和DcR 2的mRNA在所有研究年龄的大鼠睾丸中均存在，但从青春期到成年期，DcR 2/TRAIL-R4 mill水平较高。总之，本研究结果表明：1）TRAIL及其受体在正常发育过程中在睾丸中表达，2）TRAIL蛋白存在于不同的生殖细胞类型中，而其受体主要在减数分裂后的生殖细胞中检测到。
Tumor necrosis factor-alpha-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor-alpha family of cytokines that is known to induce apoptosis upon binding to its death domain-containing receptors, DR4/TRAIL-R1 and DR5/TRAIL-R2. Two additional TRAIL receptors, DcR1/TRAIL-R3 and DcR2/TRAIL-R4, lack functional death domains and act as decoy receptors for TRAIL. In this study, the presence of TRAIL and its receptors was investigated in the rat testis during development. TRAIL and its receptors were immunolocalized to the different testicular cell types. TRAIL and its receptors were also identified in the rat testis in terms of protein and mRNA. Our immunohistochemical studies indicate that TRAIL, DR5/TRAIL-R2, and DcR2-TRAIL-R4 are detected in Leydig cells, whereas ligand and all receptors are localized in germ cells. TRAIL was permanently immunodetected in germ cells from the fetal stage to adulthood, whereas its receptors were immunolocalized exclusively in postmeiotic germ cells. The expression of TRAIL and receptor mRNAs was consistent with the immunodetection of TRAIL and receptor proteins. Indeed, TRAIL ligand mRNA was also identified in the rat testis from the fetal stage to adulthood. The mRNAs of the death receptors, DR4/TRAIL-R1 and DR5/TRAIL-R2, were weakly detected during the perinatal period and increased from the pubertal stage to adulthood. The mRNAs of the decoy receptors, DcR1 and DcR2, were present in the rat testis at all ages studied, but the DcR2/TRAIL-R4 mill level was higher from the pubertal period to adulthood. Together, the present findings demonstrate that 1) TRAIL and its receptors are expressed in the testis during normal development, and 2) TRAIL protein is present in the different germ cell types, whereas its receptors were predominantly detected in the postmeiotic germ cells.