PATTERNS OF GLUTAMATE-DECARBOXYLASE IMMUNOSTAINING IN THE FELINE COCHLEAR NUCLEAR-COMPLEX STUDIED WITH SILVER ENHANCEMENT AND ELECTRON-MICROSCOPY
PATTERNS OF GLUTAMATE-DECARBOXYLASE IMMUNOSTAINING IN THE FELINE COCHLEAR NUCLEAR-COMPLEX STUDIED WITH SILVER ENHANCEMENT AND ELECTRON-MICROSCOPY
复制标题
DOI：
10.1002/cne.902620305
复制
发表时间：
1987-08-15
影响因子：
2.5
通讯作者：
MUGNAINI, E
中科院分区：
文献类型：
--
作者：
ADAMS, JC;MUGNAINI, E
使用谷氨酸脱羧酶（GAD）（抑制性神经递质 GABS 的生物合成酶）的抗血清对猫的耳蜗核复合体进行免疫染色，并使用不同的程序进行研究，包括银强化、局部秋水仙碱注射、半薄切片和免疫电子显微镜。在细胞核的所有部分都发现了免疫染色。观察到的免疫染色细胞体相对较少：大多数位于耳蜗背核，包括星状细胞、车轮细胞、高尔基细胞和深层不明细胞。在小细胞帽内和沿着腹侧鳕鱼核的内侧边界还发现了免疫反应细胞的积累。腹侧核的大细胞部分中免疫染色细胞稀疏。细胞核内的大部分染色是神经末梢的染色。这些包括小纽扣，这些小纽扣在耳蜗背核的神经毡、颗粒细胞域、小细胞帽区域内表面颗粒细胞层下方的区域以及沿着腹侧核的内侧边界中突出。章鱼细胞显示出小的 GAD 阳性末端，以中等密度分布在细胞体和树突上。在腹侧核的大细胞上，特别是球形细胞和球状细胞上，发现了更大、更独特的末端。 GAD 阳性末端的大小、位置和复杂性与相同细胞上初级纤维末端的大小、位置和复杂性之间存在显着的正相关性。这种相关性在背核中并不存在，尽管锥体细胞的细胞体上缺乏初级末梢，但背核中的锥体细胞接收到许多大的GAD阳性体细胞末端。 GAD阳性末端包含多形性突触小泡并形成对称突触连接，其占据细胞体、树突、轴突小丘和初始轴突片段的起始部分的并置表面的大量蛋白质。因此，具有大末端的细胞可能会受到相当大的抑制，这种抑制可能通过初级纤维和中间神经元或通过来自听觉脑干的下行输入间接激活。
The cochlear nuclear complex of the cat was immunostained with an antiserum to glutamate decarboxylase (GAD), the biosynthetic enzyme for the inhibitory neurotransmitter GABS, and studied with different procedures, including silver intensification, topical colchicine injections, semithin sections, and immunoelectron microscopy. Immunostaining was found in all portions of the nucleus. Relatively few immunostained cell bodies were observed: most of these were in the dorsal cochlear nucleus and included stellate cells, cartwheel cells, Golgi cells, and unidentified cells in the deep layers. An accumulation of immunoreactive cells was also found within the small cell cap and along the medial border of the ventral codhlear nucleus. Immunostained cells were sparse in magnocellular portions of the ventral nucleus. Most staining within the nucleus was of nerve terminals. These included smll boutons that were prominent in the neuropil of the dorsal cochlear nucleus, the granule cell domain, in a region beneath the superficial granule cell layer within the small cell cap region, and along the medial border of the ventral nucleus. Octopus cells showed small, GAD-positive terminals distributed at moderate density on both cell bodies and dendrities. Larger more distinctive terminals were identified on the large cells in the ventral nucleus, in particular on spherical cells and globular cells. There was a striking positive correlation of the size, location, and complexity of GAD-positive terminals with the size, location, and complexity of primary fiber endings on the same cells. This correlation did not hold in the dorsal nucleus, where pyramidal cells receive many large GAD-positive somatic terminals despite the paucity of primary endings on their cell bodies. The GAD-positive terminals contained pleomorphic synaptic vesicles and formed symmetric synaptic junctions that occupied a substantial proteion of the appositional surface to cell bodies, dendrites, axon hillocks, and the beginning portion of the initial axon segments. Thus, the cells provided with large terminals can be subjected to considerable inhibition that may be activated indirectly through primary fibers and interneurons or by descending inputs from the auditory brainstem.