STIMULATION OF INTESTINAL MUCOSAL GROWTH WITH INTRACOLONIC INFUSION OF SHORT-CHAIN FATTY-ACIDS
STIMULATION OF INTESTINAL MUCOSAL GROWTH WITH INTRACOLONIC INFUSION OF SHORT-CHAIN FATTY-ACIDS
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DOI：
10.1177/0148607189013002109
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发表时间：
1989-03-01
影响因子：
3.4
通讯作者：
ROMBEAU, JL
中科院分区：
文献类型：
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作者：
KRIPKE, SA;FOX, AD;ROMBEAU, JL
刺激肠粘膜生长的膳食纤维被大鼠后肠中的厌氧细菌发酵成短链脂肪酸（SCFA）乙酸盐、丙酸盐和丁酸盐。丁酸盐是体外结肠细胞的首选氧化燃料，并且提供优选的肠道燃料已被证明可以刺激体内粘膜增殖。这项研究确定了在缺乏正常 SCFA 来源（盲肠中的纤维发酵）的动物中，长期结肠输注丁酸盐或 SCFA 组合是否会刺激肠粘膜生长。成年雄性斯普拉格-道利大鼠喂食无脂肪和无纤维的元素流质饮食，并接受盲肠切除术、回结肠吻合术和插入近端结肠输注导管。然后分配大鼠接受连续输注丁酸盐（20 mM、40 mM 或 150 mM）、SCFA（70 mM 乙酸盐 + 35 mM 丙酸盐 + 20 mM 丁酸盐）或盐水，或不接受输注。第七组接受近端结肠横切和再吻合术。 7天后，分析空肠、回肠和近端结肠段的粘膜重量、蛋白质、RNA和DNA。在结肠中，相对于所有三个对照组、盐水输注组、不输注组和横断组，40 mM 丁酸盐输注导致所有粘膜参数显着升高。与对照组相比，20 mM 丁酸盐组和 SCFA 组的结肠粘膜 DNA 均有所增加。在空肠和回肠中，SCFA 组的粘膜 DNA 含量显着高于对照组。这些数据表明，注入大鼠结肠的 SCFA 在整个肠道中具有营养作用，而丁酸盐似乎在结肠局部具有其主要营养作用。
Dietary fiber, which stimulates intestinal mucosal growth, is fermented by anaerobic bacteria in the rat hindgut to the short-chain fatty acids (SCFA) acetate, propionate, and butyrate. Butyrate is the preferred oxidative fuel of the colonocyte in vitro, and the provision of preferred intestinal fuels has been shown to stimulate mucosal proliferation in vivo. This study determined whether chronic colonic infusion of butyrate or a combination of SCFA would stimulate intestinal mucosal growth in an animal deprived of its normal source of SCFA, fiber fermentation in the cecum. Adult male Sprague-Dawley rats were fed a fat- and fiber-free elemental liquid diet and underwent cecectomy, ileocolic anastomosis, and insertion of a proximal colonic infusion catheter. Rats were then assigned to receive either a continuous infusion of butyrate (20 mM, 40 mM, or 150 mM), SCFA (70 mM acetate + 35 mM propionate + 20 mM butyrate), or saline, or to receive no infusion. A seventh group underwent proximal colonic transection and reanastomosis. After 7 days, jejunal, ileal, and proximal colonic segments were analyzed for mucosal weight, protein, RNA, and DNA. In the colon, the 40-mM butyrate infusion resulted in significant elevations n all mucosal parameters relative to all three control groups, saline infusion, no infusion, and transection. Both the 20-mM butyrate and the SCFA groups showed increased colonic mucosal DNA compared to controls. In the jejunum and ileum, mucosal DNA content was significantly greater in the SCFA group than in the control groups. These data indicate that SCFA infused into the rat colon has a trophic effect throughout the intestinal tract, whereas butyrate appears to have its primary trophic effect locally in the colon.