Expression of the IP3R1 Promoter-Driven nls-LacZ Transgene in Purkinje Cell Parasagittal Arrays of Developing Mouse Cerebellum
Expression of the IP3R1 Promoter-Driven nls-LacZ Transgene in Purkinje Cell Parasagittal Arrays of Developing Mouse Cerebellum
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DOI：
10.1002/jnr.22451
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发表时间：
2010-10-01
影响因子：
4.2
通讯作者：
Furuichi, Teiichi
中科院分区：
文献类型：
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作者：
Furutama, Daisuke;Morita, Noriyuki;Furuichi, Teiichi
小脑浦肯野细胞单层被组织成具有不同分子组成的异质性浦肯野细胞隔室。在这里，我们描述了一个转基因小鼠系，1 NM 13，显示异质性的转基因表达在parasystrophic浦肯野细胞阵列。该转基因由核定位信号（nls）融合到由1型肌醇1，4，5-三磷酸受体（IP 3R 1）基因启动子驱动的β-半乳糖苷酶（lacZ）复合基因组成。IP（3）R1-nls-lacZ转基因表达在单个浦肯野细胞水平上在整个安装的X-gal染色的小脑表面上检测到，因为nls-lacZ活性的核积累。发育中的1 NM 13小鼠小脑显示出条带状的浦肯野细胞亚群的X-gal染色模式。X-gal条纹图案可能早在E15就由内在特性决定，并随着小脑发育而显示出越来越复杂的性质。X-gal条纹图案使人想起，但不完全相同，条纹图案的zeolite II免疫反应性。我们将对称的X-gal阳性（转基因阳性，Tg（+））中线周围浦肯野细胞条纹为Vermal Tg 1（+），Tg 2（a，B）（+）和Tg 3（a，B）（+）条纹和半球形Tg 4（a，B）（+），Tg 5（a，B）（+），Tg 6（a，B，c）（+）和Tg 7（a，B）（+）条纹，其中a、B和c表示子条纹。我们还指定了三个副花斑子条纹Tg 8（a，B，c）。P5时X-gal条纹的边界与颗粒细胞迁移通过的浦肯野细胞层中的中缝一致，表明X-gal条纹与中缝形成可能相关。我们的研究结果表明，1 NM 13是一个很好的小鼠模型与浦肯野细胞阵列的区室化的可重复性和明确的标记。(C)2010 Wiley-Liss，Inc.
The cerebellar Purkinje cell monolayer is organized into heterogeneous Purkinje cell compartments that have different molecular compositions. Here we describe a transgenic mouse line, 1NM13, that shows heterogeneous transgene expression in parasagittal Purkinje cell arrays. The transgene consists of a nuclear localization signal (nls) fused to the beta-galactosidase (lacZ) composite gene driven by the type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) gene promoter. IP(3)R1-nls-lacZ transgene expression was detected at a single Purkinje cell level over the surface of a whole-mount X-gal-stained cerebellum because of nuclear accumulation of the nls-lacZ activity. Developing cerebella of 1NM13 mice showed stripe-like X-gal staining patterns of parasagittal Purkinje cell subsets. The X-gal stripe pattern was likely determined by an intrinsic property as early as E15 and showed increasing complexity with cerebellar development. The X-gal stripe pattern was reminiscent of, but not identical to, the stripe pattern of zebrin II immunoreactivity. We designated the symmetrical X-gal-positive (transgene-positive, Tg(+)) Purkinje cell stripes about the midline as vermal Tg1(+), Tg2(a, b)(+) and Tg3(a, b)(+) stripes and hemispheric Tg4(a, b)(+), Tg5(a, b)(+), Tg6(a, b, c)(+) and Tg7(a, b)(+) stripes, where a, b, and c indicate substripes. We also assigned three parafloccular substripes Tg8(a, b, c). The boundaries of X-gal stripes at P5 were consistent with raphes in the Purkinje cell layer through which granule cells migrate, suggesting a possible association of the X-gal stripes with raphe formation. Our results indicate that 1NM13 is a good mouse model with a reproducible and clear marker for the compartmentalization of Purkinje cell arrays. (C) 2010 Wiley-Liss, Inc.