The entorhinal cortex plays a role in extinction
The entorhinal cortex plays a role in extinction
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DOI：
10.1016/j.nlm.2005.10.001
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发表时间：
2006-03-01
影响因子：
2.7
通讯作者：
Izquierdo, I
中科院分区：
文献类型：
--
作者：
Bevilaqua, LR;Bonini, JS;Izquierdo, I
在这项研究中，我们分析了内嗅皮层的参与灭绝的学习厌恶反应。在一次试验的降压抑制性回避任务（IA）中训练具有针对内嗅皮质的输注插管的大鼠，并在没有足部电击的情况下进行连续四次每日测试，该程序诱导对照动物的条件反应消退。当以能够阻断IA记忆巩固的剂量在第一次消退期后立即注入内嗅皮层时，NMDA受体拮抗剂AP 5（25 nmol/侧），蛋白质合成抑制剂茴香霉素（300 nmol/侧）和CaMKII抑制剂KN-93（10 nmol/侧），而不是MEK 1/2抑制剂PD-98059（5 nmol/侧）阻碍IA反应的消退。当第一次和第二次测试之间的间隔为48小时而不是24小时时，获得了相同的结果。数据表明，NMDA受体的正常功能，以及CaMKII活性和蛋白质合成是必要的内嗅皮层在第一次测试期间产生灭绝的时间。我们的研究结果还表明，ERK 1/2通路在这一过程中不起作用。(c)2005年爱思唯尔公司All rights reserved.
In this study, we analyzed the participation of the entorhinal cortex in extinction of a learned aversive response. Rats with infusion cannulae aimed to the entorhinal cortex were trained in a one-trial step-down inhibitory avoidance task (IA) and submitted to four consecutive daily test sessions without the footshock, a procedure that induced extinction of the conditioned response in control animals. When infused into the entorhinal cortex immediately after the first extinction session at doses able to block consolidation of IA memory, the NMDA receptor antagonist, AP5 (25 nmol/side ), the inhibitor of protein synthesis anisomycin (300 nmol/side) and the inhibitor of CaMKII, KN-93 (10 nmol/side), but not the MEK 1/2 inhibitor PD-98059 (5 nmol/side) hindered extinction of the IA response. The same results were obtained when the interval between the first and second test session was 48 instead of 24 h. The data indicate that normal functionality of the NMDA receptors, together with CaMKII activity and protein synthesis are necessary in the entorhinal cortex at the time of the first test session to generate extinction. Our results also Suggest that the ERK1/2 pathway does not play a role in this process. (c) 2005 Elsevier Inc. All rights reserved.