The fundamental properties of the fibroblast and the macrophage. II. The macrophage.
The fundamental properties of the fibroblast and the macrophage. II. The macrophage.
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DOI：
10.1084/jem.44.3.285
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发表时间：
1926-09-01
影响因子：
15.3
通讯作者：
Ebeling, AH
中科院分区：
文献类型：
--
作者：
Carrel, A;Ebeling, AH
在纯培养物中比较来自皮下结缔组织的巨噬细胞与来自血液的单核细胞：（1）将两者置于相同组成的培养基中;（2）在不同组成的培养基中研究两者，以确定代谢变化是否改变细胞质结构。观察了运动方式、动质结构、菌落特征、食物需求和对氧化砷的敏感性。结果表明：（1）单核细胞和巨噬细胞在相同的条件下生长时，其外观是相同的;（2）某些胞质结构的形式取决于培养液的组成和细胞的代谢状态;（3）两种细胞在纯培养物中生长时，其某些确定的生理特性是明显的;（4）了解这些基本特征可以解释这些细胞在生物体内的行为。
Macrophages derived from subcutaneous connective tissue were compared in pure cultures with monocytes from blood by: (1) placing both in media of identical composition; (2) studying both in media of differing composition to determine whether metabolic changes modify cytoplasmic structure. Observations were made on mode of locomotion, structure of kinoplasm, characteristics of colonies, food requirements, and susceptibility to arsenious oxide. It was found that (1) monocytes and macrophages become identical in appearance when they live under identical conditions; (2) the form of certain cytoplasmic structures depends on the composition of culture fluid and on the metabolic state of the cells; (3) certain definite physiological properties of both kinds of cells become apparent when grown in pure cultures; (4) knowledge of these fundamental characteristics explains the behavior of these cells within the organism.