TEMPORAL ASPECTS OF DNA AND RNA-SYNTHESIS DURING HUMAN IMMUNODEFICIENCY VIRUS-INFECTION - EVIDENCE FOR DIFFERENTIAL GENE-EXPRESSION
TEMPORAL ASPECTS OF DNA AND RNA-SYNTHESIS DURING HUMAN IMMUNODEFICIENCY VIRUS-INFECTION - EVIDENCE FOR DIFFERENTIAL GENE-EXPRESSION
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DOI：
10.1128/jvi.63.9.3708-3713.1989
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发表时间：
1989-09-01
影响因子：
5.4
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
KIM, SY;BYRN, R;BALTIMORE, D
逆转录病毒DNA和RNA合成的动力学是理解病毒生长的重要参数，特别是对于编码其自身的几个调控基因的人类免疫缺陷病毒（HIV）。我们已经建立了HIV在H9细胞（一种人CD 4+淋巴细胞系）中的单周期生长条件。感染后4 h可首次检测到全长病毒线性DNA。在HIV的一步生长过程中，病毒DNA的量逐渐增加，直到感染后8至12小时，然后减少。未整合的病毒DNA的拷贝数即使在其峰值也不是特别高。最引人注目的是，有一个时间程序的RNA积累：最早的RNA是大大丰富的2-脱氢酶亚基因组的mRNA种类，而9.2-脱氢酶RNA，这是基因组RNA和mRNA的水平保持低，直到感染后24小时。病毒在感染后约24小时开始产生。因此，病毒DNA合成与其他逆转录病毒一样快，但病毒RNA合成涉及积累的物种的时间改变，推测是病毒调节基因的结果。
The kinetics of retroviral DNA and RNA synthesis are parameters vital to understanding viral growth, especially for human immunodeficiency virus (HIV), which encodes several of its own regulatory genes. We have established a single-cycle growth condition for HIV in H9 cells, a human CD4+ lymphocyte line. The full-length viral linear DNA is first detectable by 4 h postinfection. During a one-step growth of HIV, amounts of viral DNA gradually increase until 8 to 12 h postinfection and then decrease. The copy number of unintegrated viral DNA is not extraordinarily high even at its peak. Most strikingly, there is a temporal program of RNA accumulation: the earliest RNA is greatly enriched in the 2-kilobase subgenomic mRNA species, while the level of 9.2-kilobase RNA which is both genomic RNA and mRNA remains low until after 24 h of infection. Virus production begins at about 24 h postinfection. Thus, viral DNA synthesis is as rapid as for other retroviruses, but viral RNA synthesis involves temporal alteration in the species that accumulate, presumably as a consequence of viral regulatory genes.