Excretory/secretory products from two Fasciola hepatica isolates induce different transcriptional changes and IL-10 release in LPS-activated bovine "BOMA" macrophages
Excretory/secretory products from two Fasciola hepatica isolates induce different transcriptional changes and IL-10 release in LPS-activated bovine "BOMA" macrophages
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DOI：
10.1007/s00436-017-5588-6
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发表时间：
2017-10-01
影响因子：
2
通讯作者：
Januszkiewicz, Kamil
中科院分区：
文献类型：
--
作者：
Baska, Piotr;Norbury, Luke James;Januszkiewicz, Kamil
肝片吸虫是一种寄生在哺乳动物胆管中的吸虫。感染每年在动物生产中造成30亿美元的损失，被认为是一种日益重要的人畜共患病。在肝片吸虫研究中，一个代表性不足的领域是检查不同寄生虫分离株对宿主免疫系统的不同免疫调节能力。本文以牛巨噬细胞系“BOMA”为研究对象，对这一问题进行了探讨。细胞经内毒素处理成熟后，用来自肝片吸虫实验室分离株“Weybridge”(fh-weyes)和野生分离株(fh-Wildes)的排泄/分泌抗原(ES)刺激。正如预期的那样，成分高度相似的抗原混合物的刺激导致了轻微的转录差异。然而，在细胞因子水平上存在显著差异。与fh-weyes相比，接触fh-Wildes上调了27个基因，下调了30个基因。两个分离株的Fh-ES均可减少肿瘤坏死因子-α的释放，而只有Fh-Wildes可减少IL-10的分泌。Fh-weyes和Fh-Wildes对IL-12的释放均无影响。我们的结果表明，不同的菌株可以有不同的免疫调节能力和对牛免疫系统的影响。
Fasciola hepatica are trematodes that reside in the bile ducts of mammals. Infection causes US$3 billion in losses annually in animal production and is considered a zoonosis of growing importance. An under-represented area in F. hepatica research has been the examination of the different immunomodulatory abilities of various parasite isolates on the host immune system. In this paper, this issue was explored, with the bovine macrophage cell line "BOMA". The cells were matured by LPS treatment and stimulated with excretory/secretory antigens (ES) from two Fasciola hepatica isolates: a laboratory isolate "Weybridge" (Fh-WeyES) and a wild isolate (Fh-WildES). As expected, stimulation with antigen mixtures with highly similar compositions resulted in mild transcriptomic differences. However, there were significant differences in cytokine levels. Compared to Fh-WeyES, exposure to Fh-WildES upregulated 27 and downregulated 30 genes. Fh-ES from both isolates diminished the release of TNF-alpha, whereas only Fh-WildES decreased IL-10 secretion. Neither Fh-WeyES nor Fh-WildES had an impact on IL-12 release. Our results indicate that various isolates can have different immunomodulatory abilities and impacts on the bovine immune system.