Transcriptional activation by AP-2α is modulated by the oncogene DEK
Transcriptional activation by AP-2α is modulated by the oncogene DEK
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DOI：
10.1093/nar/gkg247
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发表时间：
2003-03-01
影响因子：
14.9
通讯作者：
Vázquez, J
中科院分区：
文献类型：
--
作者：
Campillos, M;García, MA;Vázquez, J
细胞分化和发育是在转录水平上高度调控的过程。调节这些过程的主要转录因子之一是AP-2 α，一种脊椎动物发育和胚胎发生所需的细胞类型特异性蛋白。AP-2 α还通过与癌基因c-Myc相互作用来调节细胞凋亡和细胞周期特异性事件。在寻找新型AP-2 α相互作用因子的过程中，我们使用亲和层析方法观察到癌蛋白DEK在体外与AP-2 α相互作用。在细胞培养物中AP-2 α和DEK之间存在相互作用，这通过标记的AP-2 α形式的表达以及随后的免疫检测来证明。通过使用APOE启动子活性报告基因的瞬时共表达实验，我们发现DEK刺激AP-2 α的反式激活活性超过APOE启动子。最后，电泳迁移率变动分析表明，DEK增强AP-2 α的DNA结合活性。我们的数据表明DEK作为转录共激活因子的一种新的细胞功能。
Cell differentiation and development are highly regulated processes at the transcriptional level. One of the main transcription factors that regulate these processes is AP-2alpha, a cell-type specific protein required for vertebrate development and embryogenesis. AP-2alpha also regulates apoptosis and cell-cycle specific events by interacting with the oncogene c-Myc. In searching for novel AP-2alpha- interacting factors, using an affinity chromatography approach, we have observed that oncoprotein DEK interacts with AP-2alpha in vitro. The existence of an interaction between AP-2alpha and DEK in cellular cultures was demonstrated by expression of a tagged AP-2alpha form followed by immunodetection. By transient co-expression experiments using a reporter for APOE promoter activity we have found that DEK stimulates the transactivation activity of AP-2alpha over APOE promoter. Finally, electrophoretic mobility shift assays suggested that DEK enhances the DNA-binding activity of AP-2alpha. Our data suggest a novel cellular function of DEK as a transcriptional co-activator.