NEURONAL DETERMINATION WITHOUT CELL-DIVISION IN XENOPUS EMBRYOS
NEURONAL DETERMINATION WITHOUT CELL-DIVISION IN XENOPUS EMBRYOS
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DOI：
10.1016/0896-6273(91)90053-3
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发表时间：
1991-04-01
期刊：
影响因子：
16.2
通讯作者：
HARTENSTEIN, V
中科院分区：
文献类型：
--
作者：
HARRIS, WA;HARTENSTEIN, V
通过在 DNA 合成抑制剂羟基脲和阿菲迪霉素的混合物中孵育胚胎，可以阻断非洲爪蟾 CNS 中的细胞分裂。 令人惊讶的是，在原肠胚形成开始时接受处理的胚胎正常地进行神经形成、神经管闭合和中枢神经系统细分。 因此，细胞分裂对于中枢神经系统的神经诱导或早期形态发生事件并不重要。 处理过的胚胎中的神经母细胞分化为许多类别的神经元，这表明中枢神经系统中的细胞决定可以与谱系和出生日期无关。 轴突束和胚胎反射也发育起来。 这些动物中发生的大量正常中枢神经系统发育可以通过一系列很大程度上独立于细胞增殖的连续诱导来解释。
Cell division in the Xenopus CNS was blocked by incubating embryos in a mixture of the DNA synthesis inhibitors hydroxyurea and aphidicolin. Surprisingly, embryos treated at the beginning of gastrulation proceeded normally through neurulation, neural tube closure, and CNS subdivision. Thus, cell division is not critical for neural induction or early morphogenetic events in the CNS. Neuroblasts in treated embryos differentiated into neurons of many classes, indicating that cellular determination in the CNS can be dissociated from lineage and birth date. Axonal tracts and embryonic reflexes also developed. The remarkable amount of normal CNS development that occurs in these animals may be explained by a series of sequential inductions that are largely independent of cell proliferation.