Transcriptome analysis of NF-κB- and phosphatidylinositol 3-kinase-regulated genes in human cytomegalovirus-infected monocytes
Transcriptome analysis of NF-κB- and phosphatidylinositol 3-kinase-regulated genes in human cytomegalovirus-infected monocytes
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DOI：
10.1128/jvi.00864-07
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发表时间：
2008-01-01
影响因子：
5.4
通讯作者：
Yurochko, Andrew D.
中科院分区：
文献类型：
--
作者：
Chan, Gary;Bivins-Smith, Elizabeth R.;Yurochko, Andrew D.
人巨细胞病毒在感染后诱导单核细胞的促炎性，我们有证据表明NF-κ B B和磷脂酰肌醇3-激酶[PI（3）K]是这种早期激活的关键介质。为了开始解决这些信号通路如何负责感染的单核细胞的快速活化，我们检查了这些通路在感染的单核细胞的转录组中所起的作用。使用cDNA微阵列进行的全局转录谱分析显示，包括炎症基因在内的大量基因以NF-κ B和/或PI（3）K依赖性方式受到调控，确定NF-κ B和PI（3）K途径是HCMV感染后单核细胞转化为活化促炎状态的关键细胞控制点。
Human cytomegalovirus induces a proinflammatory monocyte following infection, and we have evidence that NF-kappa B and phosphatidylinositol 3-kinase [PI(3)K] are key mediators in this early activation. To begin to address how these signaling pathways are responsible for the rapid activation of infected monocytes, we examined the role that these pathways played in the transcriptome of infected monocytes. Global transcriptional profiling using cDNA microarrays revealed that a significant number of genes, including inflammatory genes, were regulated in an NF-kappa B- and/or PI(3)K-dependent manner, identifying the NF-kappa B and PI(3)K pathways as key cellular control points in the conversion of monocytes to an activated proinflammatory state following HCMV infection.