Resolving species-level diversity of Beringiana and Sinanodonta mussels (Bivalvia: Unionidae) in the Japanese archipelago using genome-wide data
Resolving species-level diversity of Beringiana and Sinanodonta mussels (Bivalvia: Unionidae) in the Japanese archipelago using genome-wide data
复制标题
使用全基因组数据解决日本群岛 Beringiana 和 Sinanodonta 贻贝（双壳纲：Unionidae）的物种水平多样性
DOI：
10.1016/j.ympev.2022.107563
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发表时间：
2022
影响因子：
4.1
通讯作者：
Chiba Satoshi
中科院分区：
文献类型：
--
作者：
Sano Isao;Saito Takumi;Ito Shun;Ye Bin;Uechi Takeru;Seo Tomoki;Do Van Tu;Kimura Kazuki;Hirano Takahiro;Yamazaki Daishi;Shirai Akihisa;Kondo Takaki;Miura Osamu;Miyazaki Jun-Ichi;Chiba Satoshi
准确的物种识别在生态学和进化生物学中具有重要意义。很长一段时间以来，蚌类贝灵根蚌和Sinanodonta蚌一直困扰着试图揭示它们多样性的研究人员，因为它们难以辨别的形态。最近的一项研究基于线粒体DNA变异对蚌类进行了物种划分，为掌握蚌类的物种多样性开辟了新的途径。然而，基于mtDNA的分类可能与物种边界不一致，因为mtDNA容易渗入和不完全的谱系分类，导致物种隶属关系和基因同源性之间的不一致。在这项研究中，我们评估了基于线粒体DNA的分类unionid musselsBeringiana和Sinanodontain日本使用线粒体序列数据，双酶切限制性位点相关DNA文库（ddRAD）测序，和形态学数据的有效性。我们发现线粒体和核DNA的同源性存在显著的不一致性，这使我们对这一组中基于mtDNA的分类的可靠性产生了怀疑。此外，核DNA系统发育研究表明，线粒体DNA系统发育中至少隐藏着两个蚌类谱系。尽管分子测年技术表明Beringiana-Sinanodonta在3500万年前就已经分化，但它们的外壳形态往往难以区分。具体而言，形态分析显示，在两个属在琵琶湖，这进一步复杂的物种识别和蚌类贻贝的形态进化几乎相同的球状壳形式的平行外观。我们的研究增加了越来越多的文献，准确的蚌类的物种识别时，单独使用形态特征是困难的。虽然基于线粒体DNA的分类是一种简单方便的方法来分类蚌类，相当谨慎的应用在生态和进化研究。
Accurate species identification is of primary importance in ecology and evolutionary biology. For a long time, the unionid musselsBeringianaandSinanodontahave puzzled researchers trying to unravel their diversity because of their poorly discernible morphologies. A recent study conducted species delineation of unionid mussels based on mitochondrial DNA variation, opening up a new avenue to grasp species diversity of the mussels. However, mtDNA-based classification may not align with species boundaries because mtDNA is prone to introgression and incomplete lineage sorting that cause discordance between species affiliation and gene phylogeny. In this study, we evaluated the validity of the mtDNA-based classification of unionid musselsBeringianaandSinanodontain Japan using mitochondrial sequence data, double digest restriction site-associated DNA library (ddRAD) sequencing, and morphological data. We found significant inconsistencies in the mitochondrial and nuclear DNA phylogenies, casting doubt on the reliability of the mtDNA-based classification in this group. In addition, nuclear DNA phylogeny revealed that there are at least two unionid lineages hidden in the mtDNA phylogeny. Although molecular dating technique indicates thatBeringianaandSinanodontadiverged >35 million years ago, their shell morphologies are often indistinguishable. Specifically, morphological analyses exhibited the parallel appearance of nearly identical ball-like shell forms in the two genera in Lake Biwa, which further complicates species identification and the morphological evolution of unionid mussels. Our study adds to a growing body of literature that accurate species identification of unionid mussels is difficult when using morphological characters alone. Although mtDNA-based classification is a simple and convenient way to classify unionid mussels, considerable caution is warranted for its application in ecological and evolutionary studies.