Evolution of estrogen receptors in ray-finned fish and their comparative responses to estrogenic substances
Evolution of estrogen receptors in ray-finned fish and their comparative responses to estrogenic substances
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DOI：
10.1016/j.jsbmb.2015.12.009
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发表时间：
2016-04
期刊：
影响因子：
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通讯作者：
Saki Tohyama;S. Miyagawa;A. Lange;Y. Ogino;Takeshi Mizutani;M. Ihara;Hiroaki Tanaka;N. Tatarazako;Tohru Kobayashi;C. Tyler;T. Iguchi
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文献类型：
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作者：
Saki Tohyama;S. Miyagawa;A. Lange;Y. Ogino;Takeshi Mizutani;M. Ihara;Hiroaki Tanaka;N. Tatarazako;Tohru Kobayashi;C. Tyler;T. Iguchi
在脊椎动物中，雌激素通过雌激素受体(ESRS)在调节生殖活动中起着基础性的作用，雌激素信号的干扰现在是野生动物和人类健康共同关注的问题。到目前为止，仅对有限数量的物种的ESRS进行了表征。我们研究了脊椎动物中最易变的类群--放射鳍鱼类(Actinopterygii)中ESRS的功能多样性和分子基础或配体敏感性。我们克隆并鉴定了鳍鱼进化中的几个关键物种的ESRS，包括位于鳍鱼基础谱系的Bichir(多翅目，ESR1和ESR2)，以及龙鱼(骨舌形目，ESR1和ESR2b)和鳗鱼(鳗形目，ESR1，ESR2a和ESR2b)，这两个物种都属于硬骨鱼的早期分支谱系，表明ESR2a和ESR2b是通过硬骨鱼特有的全基因组复制出现的，但ESR1的同源序列在Euteleost物种的早期谱系中已经丢失。所有克隆的ESR亚型对内源性和合成类固醇雌激素的反应相似，但对非类固醇类雌激素内分泌干扰物(EDCs)的反应不同(例如，与ESR2b相比，ESR2a表现出较弱的报告活性)。我们发现ESRS的配体敏感性的差异可以归因于鳍鱼不同分类群物种之间的系统发育。所提供的分子信息有助于了解ESRS在鱼类生殖生物学中的比较作用及其对EDCs的比较反应。
In vertebrates, estrogens play fundamental roles in regulating reproductive activities through estrogen receptors (ESRs), and disruption of estrogen signaling is now of global concern for both wildlife and human health. To date, ESRs of only a limited number of species have been characterized. We investigated the functional diversity and molecular basis or ligand sensitivity of ESRs among ray-finned fish species (Actinopterygii), the most variable group within vertebrates. We cloned and characterized ESRs from several key species in the evolution of ray-finned fish including bichir (Polypteriformes, ESR1 and ESR2) at the basal lineage of ray-finned fish, and arowana (Osteoglossiformes, ESR1 and ESR2b) and eel (Anguilliformes, ESR1, ESR2a and ESR2b) both belonging to ancient early-branching lineages of teleosts, and suggest that ESR2a and ESR2b emerged through teleost-specific whole genome duplication, but an ESR1 paralogue has been lost in the early lineage of euteleost fish species. All cloned ESR isoforms showed similar responses to endogenous and synthetic steroidal estrogens, but they responded differently to non-steroidal estrogenic endocrine disrupting chemicals (EDCs) (e.g., ESR2a exhibits a weaker reporter activity compared with ESR2b). We show that variation in ligand sensitivity of ESRs can be attributed to phylogeny among species of different taxonomic groups in ray-finned fish. The molecular information provided contributes both to understanding of the comparative role of ESRs in the reproductive biology of fish and their comparative responses to EDCs.