Single ion channel recordings with CMOS-anchored lipid membranes.
Single ion channel recordings with CMOS-anchored lipid membranes.
复制标题
使用 CMOS 锚定脂质膜进行单离子通道记录。
DOI：
10.1021/nl400822r
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发表时间：
2013
期刊：
影响因子：
10.8
通讯作者：
Shepard,KennethL
中科院分区：
文献类型：
--
作者：
Rosenstein,JacobK;Ramakrishnan,Siddharth;Roseman,Jared;Shepard,KennethL
我们提出了用仿生脂质膜进行的单离子通道记录，该脂质膜直接附着在互补金属氧化物半导体（CMOS）前置放大器芯片的表面。利用该系统，我们解决了来自几种细菌离子通道的单通道电流，包括单个alamethicin通道在1 MHz带宽下的波动，这代表了迄今为止报道的最快的单离子通道记录。该平台还用于高分辨率α-溶血素纳米孔记录。这些结果表明，通过利用集成半导体平台实现高级离子通道接口，可以实现高信号保真度，精细时间分辨率，小几何形状和多路集成。
We present single-ion-channel recordings performed with biomimetic lipid membranes which are directly attached to the surface of a complementary metal–oxide–semiconductor (CMOS) preamplifier chip. With this system we resolve single-channel currents from several types of bacterial ion channels, including fluctuations of a single alamethicin channel at a bandwidth of 1 MHz which represent the fastest single-ion-channel recordings reported to date. The platform is also used for high-resolution α-hemolysin nanopore recordings. These results illustrate the high signal fidelity, fine temporal resolution, small geometry, and multiplexed integration which can be achieved by leveraging integrated semiconductor platforms for advanced ion channel interfaces.