ELECTROPHORESIS AND DIFFUSION IN THE PLANE OF THE CELL-MEMBRANE
ELECTROPHORESIS AND DIFFUSION IN THE PLANE OF THE CELL-MEMBRANE
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DOI：
10.1016/s0006-3495(79)85231-5
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发表时间：
1979-01-01
影响因子：
3.4
通讯作者：
CHAO, AW
中科院分区：
文献类型：
--
作者：
POO, MM;LAM, JW;CHAO, AW
用显微荧光法和离子电泳法研究了刀豆蛋白A(ConA)受体和乙酰胆碱(ACh)受体在单核和球形非洲爪蛙成肌细胞质膜平面上的电泳性和弥散性运动。在沿细胞表面施加10V/cm的均匀电场时，这两种受体在30分钟内向细胞的阴极处部分聚集；电场松弛后，培养10分钟内ConA受体的均匀分布完全恢复；与一般的ConA受体相反，电场使ACh受体聚集，形成稳定的、局部的受体聚集体。对电泳膜受体在电泳法和扩散法平衡时的分布和去场后的反扩散速率进行了理论分析。这些分析表明，在22度。Con A受体的平均凝胶迁移率约为1.9倍。每V/cm 10~(-3)微米/S，平均扩散系数为5.1倍。10-9cm2/S。
Electrophoretic and diffusional movements of concanavalin A (Con A) receptors and acetylcholine (ACh) receptors in the plane of the plasma membrane of mononucleate, spherical Xenopus myoblasts were studied by microfluorimetry and iontophoresis. A uniform electric field of 10 V/cm applied along the cell surface produces a partial accumulation of both types of receptors toward the cathodal pole of the cell within 30 min; post-field relaxation of the culture results in the complete recovery of the uniform distribution of the Con A receptors within 10 min; and in contrast to the Con A receptor in general, accumulation of ACh receptors by the electric field results in the formation of stable, localized receptor aggregates. Theoretical analyses were carried out for the distribution of charged membrane receptors at equilibrium between electrophoresis and diffusion and for the rate of back diffusion after the removal of the field. These analyses indicated that, at 22.degree. C, the average electrophoretic mobility of the electrophoretically mobile population of the Con A receptors is about 1.9 .times. 10-3 .mu.m/s per V/cm, while their average diffusion coefficient is 5.1 .times. 10-9 cm2/s.