Genome sequencing and transcriptome analysis of Geotrichum citri-aurantii on citrus reveal the potential pathogenic- and guazatine-resistance related genes.
Genome sequencing and transcriptome analysis of Geotrichum citri-aurantii on citrus reveal the potential pathogenic- and guazatine-resistance related genes.
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DOI：
10.21203/rs.2.18313/v1
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发表时间：
2019-12
期刊：
影响因子：
4.4
通讯作者：
Juan Zhao;Deyao Zhang;Zhe Wang;Zhonghuan Tian;Fan Yang;Xinjun Lu;C. Long
中科院分区：
文献类型：
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作者：
Juan Zhao;Deyao Zhang;Zhe Wang;Zhonghuan Tian;Fan Yang;Xinjun Lu;C. Long
由柑桔地霉（Geotrichum citri-aurantii）引起的酸腐病是柑桔采后的一种主要病害，造成严重的经济损失。本研究利用高质量的G.通过单分子实时测序（SMRT）获得柠檬酸橙。获得了大约5.43 Gb的干净数据，并在高穿透染色体构象捕获（Hi-C）组装后将总共27.94 Mb的基因组序列映射到10个染色体组。此外，还克隆了3个与致病性相关的多聚半乳糖醛酸酶基因。Citri-aurantii基因组被发现。并对抗性基因的转录组数据进行了分析，结果表明，G.柑橘橙黄病与两个ATP结合盒（ABC）转运蛋白家族基因、六个主要易化超家族（MFS）转运蛋白家族基因和两个多药毒性化合物外排（MATE）转运蛋白家族基因相关。总之，我们的研究可能为有效控制这种病原体提供新的见解。
Sour rot, caused by Geotrichum citri-aurantii, is a major postharvest disease of citrus，and it causes serious economic losses. In this study, a high-quality genome sequence of G. citri-aurantii was obtained by Single Molecule Real-Time Sequencing (SMRT). Approximately 5.43 Gb of clean data were obtained and a total of 27.94-Mb genomic sequence was mapped to 10 chromosome groups after high-through chromosome conformation capture (Hi-C) assembly. In addition, three polygalacturonase genes which were related to pathogenicity in G. citri-aurantii genome were discovered. And transcriptome data of guazatine-resistance had been analyzed, the results showed that the guazatine-resistance of G. citri-aurantii was related to two ATP-binding cassette (ABC) transporter family genes, six major facilitator superfamily (MFS) transporter family genes and two multidrug and toxic compound extrusion (MATE) transporter family genes. In summary, our research may provide novel insights into the effective control of this pathogen.