Analysis of phosphoproteome in rice pistil
Analysis of phosphoproteome in rice pistil
复制标题
水稻雌蕊磷酸化蛋白质组分析
DOI：
10.1002/pmic.201400004
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发表时间：
2014-10-01
期刊：
影响因子：
3.4
通讯作者：
Yang, Pingfang
中科院分区：
文献类型：
--
作者：
Wang, Kun;Zhao, Yong;Yang, Pingfang
雌蕊是花的雌性生殖器官，由子房、花柱和柱头组成，是花粉识别、受精和种子形成的关键器官。以往对花粉-雌蕊互作的研究主要集中在比较转录组学或蛋白质组学方法研究基因表达的变化。然而，对蛋白质PTM的研究仍然缺乏。本文报道了水稻成熟雌蕊的磷酸蛋白质组学研究。采用IMAC富集、亲水作用层析和高准确度质谱仪（TripleTOF 5600），高置信度（Ascore >= 19，p
As the female reproductive part of a flower, the pistil consists of the ovary, style, and stigma, and is a critical organ for the process from pollen recognition to fertilization and seed formation. Previous studies on pollen-pistil interaction mainly focused on gene expression changes with comparative transcriptomics or proteomics method. However, studies on protein PTMs are still lacking. Here we report a phosphoproteomic study on mature pistil of rice. Using IMAC enrichment, hydrophilic interaction chromatography fraction and high-accuracy MS instrument (TripleTOF 5600), 2347 of high-confidence (Ascore >= 19, p