PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae.
PIM1 encodes a mitochondrial ATP-dependent protease that is required for mitochondrial function in the yeast Saccharomyces cerevisiae.
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DOI：
10.1016/s0021-9258(17)42340-4
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发表时间：
1994-01
期刊：
影响因子：
--
通讯作者：
L. van Dyck;D. Pearce;F. Sherman
中科院分区：
文献类型：
--
作者：
L. van Dyck;D. Pearce;F. Sherman
酿酒酵母中的 PIM1 核基因编码一种线粒体 ATP 依赖性蛋白酶，与大肠杆菌的 ATP 依赖性蛋白酶 La、短芽孢杆菌的 Lon 和黄色粘球菌的 ATP 依赖性蛋白酶具有超过 30% 的同一性。此外，Pim1 长 1133 个氨基酸，在 N 末端区域具有假定的线粒体输入信号。正常 PIM1+ 和缺陷 pim1-delta 菌株的酶学比较表明，ATP 依赖性蛋白酶位于线粒体基质内。 pim1-delta 菌株无法利用不可发酵底物作为唯一碳源，也无法维持功能性线粒体 DNA，表明 Pim1 蛋白酶是线粒体功能所必需的。 PIM1 mRNA 持续表达，但在热应激后表达增加，表明 Pim1 可能在热休克反应中发挥作用。
The PIM1 nuclear gene in the yeast Saccharomyces cerevisiae encodes a mitochondrial ATP-dependent protease that exhibits over 30% identity with ATP-dependent protease La from Escherichia coli, Lon from Bacillus brevis, and one from Myxococcus xanthus. In addition, Pim1 is 1133 amino acids long and has a putative mitochondrial import signal in the N-terminal region. Enzymatic comparisons of normal PIM1+ and deficient pim1-delta strains revealed that the ATP-dependent protease is located within the mitochondrial matrix. The pim1-delta strains are unable to utilize nonfermentable substrates as the sole carbon source and are unable to maintain functional mitochondrial DNA, indicating that the Pim1 protease is required for mitochondrial function. PIM1 mRNA is constitutively expressed but is increased after thermal stress, suggesting that Pim1 may play a role in the heat shock response.