Expression of transketolase-like 1 (TKTL1) and p-Akt correlates with the progression of cervical neoplasia
Expression of transketolase-like 1 (TKTL1) and p-Akt correlates with the progression of cervical neoplasia
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DOI：
10.1111/j.1447-0756.2008.00749.x
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发表时间：
2008-06-01
影响因子：
1.6
通讯作者：
Kammerer, Ulrike
中科院分区：
文献类型：
--
作者：
Kohrenhagen, Nico;Voelker, Hans U.;Kammerer, Ulrike
目的：推测糖酵解的增加对肿瘤进展过程中的能量供应至关重要。不幸的是，糖酵解与宫颈肿瘤的相关性尚不清楚，但可以肯定的是，宫颈癌显示葡萄糖膜转运蛋白的高表达，葡萄糖膜转运蛋白是葡萄糖摄取作为能量来源所必需的。转酮酶样酶1 （TKTL1）和癌基因p-Akt在肿瘤发生过程中的糖酵解中发挥重要作用。因此，我们对它们在宫颈组织中的表达感兴趣。方法：在80例经福尔马林固定、石蜡包埋的宫颈标本中检测TKTL1和p-Akt的表达，其中包括20例良性宫颈组织、20例低级别鳞状上皮内病变、20例高级别上皮内病变和20例浸润性鳞状细胞癌。结果：免疫组化分析显示，TKTL1和p-Akt的表达强度随着宫颈组织病理分级的增加而显著升高。结论：TKTL1和p-Akt在宫颈肿瘤的发展过程中发挥重要作用，可能与它们对糖酵解的影响有关。
Aim: It is supposed that increased glycolysis is crucial for the energy supply during tumor progression. Unfortunately, the relevance of glycolysis in cervical neoplasia is unknown, but what is certain is the fact that cervical cancer shows a high expression of glucose membrane transporters, which are necessary for glucose uptake as an energy source. Transketolase-like enzyme 1 (TKTL1) and the oncogene p-Akt have been described to play an important role in glycolysis during tumorigenesis. Thus, we were interested in their expression in cervical tissue.Methods: We examined the expression of TKTL1 and p-Akt in 80 formalin-fixed, paraffin-embedded cervical specimens: 20 benign cervical tissues, 20 low-grade squamous intraepithelial lesions, 20 high-grade intraepithelial lesions, and 20 invasive squamous cell carcinomas (ISCC).Results: Immunhistochemical analyses revealed that the intensity of the expression of TKTL1 and p-Akt increases significantly with an increase in the histopathological grade of cervical tissues.Conclusion: The results suggest that both TKTL1 and p-Akt play an important role in the progression of cervical neoplasia, which may be due to their impact on glycolysis.