HERPES-SIMPLEX VIRUS TYPE-1 PATHOGENICITY IN MICE EXPRESSED BY LYTIC INFECTION IN PRE-B AND B-CELLS
HERPES-SIMPLEX VIRUS TYPE-1 PATHOGENICITY IN MICE EXPRESSED BY LYTIC INFECTION IN PRE-B AND B-CELLS
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DOI：
10.1006/clin.1994.1167
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发表时间：
1994-10-01
期刊：
影响因子：
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通讯作者：
JOLICOEUR, P
中科院分区：
文献类型：
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作者：
LAMONTAGNE, L;JOLICOEUR, P
单纯疱疹病毒1型（HSV-1）感染在人类或小鼠模型中的结果可能受到宿主免疫能力的影响。在这项工作中，我们假设HSV-1感染改变了基因决定的易感小鼠的骨髓B淋巴生成。为了验证这一假设，我们将耐药的C57BL/6或易感的A/J成年小鼠腹腔感染HSV-1 （McIntyre菌株）。在感染后的不同时间，采集两株感染小鼠的脾脏和骨髓。采用单抗对末端脱氧核苷酸转移酶（TdT）、220-kDa表面膜糖蛋白（用单抗14.8检测）和细胞质（c mu）或表面（s mu） IgM链进行双免疫荧光检测。结果表明，a /J易感小鼠脾B细胞(c μ（+)、s μ(+)）和骨髓前B细胞(c μ（+)、s μ(-)）和B细胞的百分比和绝对数量均减少。对前b (14.8（+), c mu(-)）或TdT（+）前体细胞均无影响。在体内感染易感A/J小鼠的B前细胞和B细胞中检测到细胞内病毒蛋白。体外研究还显示，病毒诱导的细胞裂解发生在纯化的脾脏和骨髓前B细胞或仅来自易感A/J小鼠的B细胞中。前B细胞和B细胞的病毒趋向性是另一种免疫缺陷机制，涉及对HSV-1的遗传敏感性。(C) 1994学术出版社，Inc.
The outcome of herpes simplex virus type 1 (HSV-1) infection in human or in the murine model can be influenced by host immunocompetency. We postulate in this work that HSV-1 infection alters bone marrow B lymphopoiesis in genetically determined susceptible mice. To verify this hypothesis, resistant C57BL/6 or susceptible A/J adult mice were intraperitoneally infected with HSV-1 (McIntyre strain). At various postinfection times, spleen and bone marrow were collected from both infected mouse strains. B lineage cell subpopulations were identified by double immunofluorescence assays using mAbs to terminal deoxynucleotidyl transferase (TdT), 220-kDa surface membrane glycoprotein (detected by mAb 14.8), and cytoplasmic (c mu) or surface (s mu) IgM chains. Results revealed a decrease in both percentage and absolute number of splenic B (c mu(+), s mu(+)) cells and bone marrow pre-B (c mu(+), s mu(-)) and B cells isolated only from susceptible A/J mice. No effect was observed on pro-B (14.8(+), c mu(-)) or TdT(+) precursor cells from both mouse strains. Intracellular viral proteins were detected in pre-B and B cells in in vivo-infected susceptible A/J mice. In vitro studies also revealed that virus-induced cell lysis occurred in purified splenic and bone marrow pre-B or B cells derived only from susceptible A/J mice. Viral tropism for pre-B and B cells represents another immunodeficiency mechanism involved in the genetic sensitivity to HSV-1. (C) 1994 Academic Press, Inc.