Reconstructing the evolutionary history of the centriole from protein components
Reconstructing the evolutionary history of the centriole from protein components
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DOI：
10.1242/jcs.064873
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发表时间：
2010-05-01
影响因子：
4
通讯作者：
Gull, Keith
中科院分区：
文献类型：
--
作者：
Hodges, Matthew E.;Scheumann, Nicole;Gull, Keith
中心粒是高度保守的结构，其实现重要的细胞功能，例如纤毛和鞭毛的成核（基体功能）和组织中心粒周围物质以形成中心体。这些功能的演变可以从现存中心粒和中心体分子组分的分布推断。在这里，我们进行了一个进化分析的53蛋白已知的中心粒协会或参与纤毛相关的病理。通过将45种不同真核生物中的蛋白质分布与生物生物学联系起来，我们提供了分子证据表明，基体功能是祖先的，而中心体的存在是特定于Holozoa的。我们定义了一个祖先中心粒库存的14个核心蛋白，Polo样激酶，蛋白质与Bardet-Biedl综合征（BBS）和Meckel-Gruber综合征。我们发现，BBSome是缺席的有机体，产生纤毛只为运动，预测一个占主导地位的和古老的作用，这个复杂的感觉功能。我们还表明，不寻常的秀丽隐杆线虫的中心粒是高度不同的蛋白质组成和序列。最后，我们证明了特定中心粒蛋白的存在和眼睛的进化之间的相关性。这种相关性被用来预测蛋白质的发展中的纤毛，但不是横纹肌，眼睛。
Centrioles are highly conserved structures that fulfil important cellular functions, such as nucleation of cilia and flagella (basal-body function) and organisation of pericentriolar material to form the centrosome. The evolution of these functions can be inferred from the distribution of the molecular components of extant centrioles and centrosomes. Here, we undertake an evolutionary analysis of 53 proteins known either for centriolar association or for involvement in cilia-associated pathologies. By linking protein distribution in 45 diverse eukaryotes with organism biology, we provide molecular evidence to show that basal-body function is ancestral, whereas the presence of the centrosome is specific to the Holozoa. We define an ancestral centriolar inventory of 14 core proteins, Polo-like-kinase, and proteins associated with Bardet-Biedl syndrome (BBS) and Meckel-Gruber syndrome. We show that the BBSome is absent from organisms that produce cilia only for motility, predicting a dominant and ancient role for this complex in sensory function. We also show that the unusual centriole of Caenorhabditis elegans is highly divergent in both protein composition and sequence. Finally, we demonstrate a correlation between the presence of specific centriolar proteins and eye evolution. This correlation is used to predict proteins with functions in the development of ciliary, but not rhabdomeric, eyes.