IMMUNOGLOBULIN-GENE REARRANGEMENTS AS UNIQUE CLONAL MARKERS IN HUMAN LYMPHOID NEOPLASMS
IMMUNOGLOBULIN-GENE REARRANGEMENTS AS UNIQUE CLONAL MARKERS IN HUMAN LYMPHOID NEOPLASMS
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DOI：
10.1056/nejm198312293092601
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发表时间：
1983-01-01
影响因子：
158.5
通讯作者：
KORSMEYER, SJ
中科院分区：
文献类型：
--
作者：
ARNOLD, A;COSSMAN, J;KORSMEYER, SJ
Ig genes in their germ-line form are spearated DNA subsegments that must be joined by means of recombinations during B-cell development. Individual Ig gene rearrangements are specific for a given B cell and its progeny. Detection of such gene rearrangements by Southern hybridization is shown to provide a sensitive marker for clonality and B-cell lineage within lymphoid tissues lacking expression of definitive surface phenotypes. These genetic markers were used in 3 ways: to establish a diagnosis of lymphoma in a neoplastic disorder of uncertain cell type, to show that some lymphomas that were previously classified as being of T-cell type in fact contain monoclonal B cells and to detect clonal B-cell populations within lymphomatous tissues of uncertain immunotype and within an atypical lymphofollicular hyperplasia having no other clonal surface markers. These sensitive and unique indicators of clonality located directly at the DNA levels are capable of providing insights into the cellular origin, early dection and natural history of neoplasia.
