Design of a prototype flow microreactor for synthetic biology in vitro
Design of a prototype flow microreactor for synthetic biology in vitro
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DOI：
10.1039/c3lc50231g
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Ces, Oscar
中科院分区：
文献类型：
--
作者：
Boehm, Christian R.;Freemont, Paul S.;Ces, Oscar
作为体外合成生物学的参考平台，我们开发了一个用于酶促生物合成的流动微反应器原型。我们报告的设计，实施和计算机辅助优化的三步模型途径在微流控反应器。在对几种方法进行实验评估后，填充床格式被证明是酶区隔化的最佳选择。通过计算建模得到的优化参数集可以显著提高比基板转换效率。我们的微反应器设计为探索新的体外合成生物学解决方案提供了一个平台，用于工业生物合成。
As a reference platform for in vitro synthetic biology, we have developed a prototype flow microreactor for enzymatic biosynthesis. We report the design, implementation, and computer-aided optimisation of a three-step model pathway within a microfluidic reactor. A packed bed format was shown to be optimal for enzyme compartmentalisation after experimental evaluation of several approaches. The specific substrate conversion efficiency could significantly be improved by an optimised parameter set obtained by computational modelling. Our microreactor design provides a platform to explore new in vitro synthetic biology solutions for industrial biosynthesis.