Estrogenic activities of extracts of Chinese licorice (Glycyrrhiza uralensis) root in MCF-7 breast cancer cells
Estrogenic activities of extracts of Chinese licorice (Glycyrrhiza uralensis) root in MCF-7 breast cancer cells
复制标题
甘草根提取物对 MCF-7 乳腺癌细胞的雌激素活性
DOI：
10.1016/j.jsbmb.2008.12.019
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发表时间：
2009-02-01
影响因子：
4.1
通讯作者：
Li, Zhong
中科院分区：
文献类型：
--
作者：
Hu, Chunyan;Liu, Huaqing;Li, Zhong
尽管广泛使用的中国甘草根（甘草乌拉尔）的治疗更年期的投诉，鲜为人知的是其潜在的雌激素特性，和现有的信息相对于其对细胞增殖的影响是矛盾的，在这项研究中，雌激素特性的甘草根进行了评估，在体外使用的几个测定。甘草根DMSO提取物浓度增加对MCF-7乳腺癌细胞生长的影响是双相的。该提取物在低浓度下表现出ER依赖性的促生长作用，在高浓度下表现出ER非依赖性的抗增殖活性。在进一步的实验中，甘草根被依次提取以产生四个级分：己烷、EtOAc、甲醇和H2O。只有EtOAc浸提液对细胞增殖的影响与DMSO浸提液相似。己烷浸提液对细胞生长无影响。与此相反，甲醇和水提取物显示出ER非依赖性，生长促进作用。与其对细胞增殖的影响相似，EtOAc提取物对S期细胞周期分布和PCNA蛋白水平具有双相作用。该提取物诱导MCF-7细胞中内源性ER α的反式激活，通过诱导ER α蛋白和mRNA水平的小丑调节以及ER α靶基因pS2和GREB 1的上调来支持。这些结果表明，甘草的活性以及癌症风险增加和预防雌激素依赖性乳腺癌之间的平衡可能取决于饮食摄入量。(C)2009爱思唯尔有限公司保留所有权利。
Despite the wide use of Chinese licorice root (Glycyrrhiza uralensis) for the treatment of menopausal complaints, little is known on its potential estrogenic properties, and available information relative to its effects on cell proliferation is contradictory, In this study, the estrogenic properties of licorice root were evaluated in vitro by use of several assays. The effects of increasing concentrations of a DMSO extract of licorice root on the growth of MCF-7 breast cancer cells were biphasic. The extract showed an ER-dependent growth-promoting effect at low concentrations and an ER-independent anti-proliferative activity at high concentrations. In further experiments, licorice root was sequentially extracted to yield four fractions: hexane, EtOAc, methanol and H2O. Only the EtOAc extract had effects on cell proliferation similar to the DMSO extract. The hexane extract had no effect on cell growth. In contrast, the methanol and water extracts showed an ER-independent, growth-promoting effect. Similar to its effects on cell proliferation, the EtOAc extract had a biphasic effect on S phase cell cycle distribution and the level of PCNA protein. This extract-induced transactivation of endogenous ER alpha in MCF-7 cells, supported by inducing clown-regulation of ER alpha protein and mRNA levels, and up-regulation of ER alpha target genes pS2 and GREB1. These results suggest that the activity of licorice root and the balance between increased risk for cancer and prevention of estrogen-dependent breast cancer may depend on the amount of dietary intake. (C) 2009 Elsevier Ltd. All rights reserved.