Nonionic Surfactant Blends to Control the Size of Microgels and Their Catalytic Performance during Glycoside Hydrolyses
Nonionic Surfactant Blends to Control the Size of Microgels and Their Catalytic Performance during Glycoside Hydrolyses
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DOI：
10.1021/acscatal.0c01887
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发表时间：
2020-07
期刊：
影响因子：
12.9
通讯作者：
B. Sharma;S. Striegler
中科院分区：
文献类型：
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作者：
B. Sharma;S. Striegler
在概念研究的证明中，使用一系列衍生自吐温80和司盘80的非离子表面活性剂共混物从细乳液中的稳定液滴制备催化微凝胶。本研究的目的是优化微凝胶的催化效率，通过减小它们的粒径与表面活性剂，是定制的各自的预聚合混合物。该方法的有效性进行检查，通过评估所得到的微凝胶的催化效率相比，在十二烷基硫酸钠（SDS）离子溶液的存在下，他们的同行。获得具有亲水亲油平衡（HLB）值依赖的平均流体动力学直径在99和200 nm之间的球形颗粒。添加Cu（II）离子和所选的其它过渡金属离子活化休眠催化剂，用于在pH 7.00和37 °C下使用荧光模型底物在2-[4-（2-羟乙基）哌嗪-1-基]哌嗪-1-基]乙磺酸（HEPES）缓冲液中裂解糖苷键。最高的催化水解的熟练度观察到的Cu的？（II）-含有微凝胶（kcat/KM × knon = 870 000）与最低的直径表明一个几乎两倍更好的稳定的过渡态相比，在离子SDS溶液的存在下制备的微凝胶。该研究建立了材料合成过程中使用的非离子表面活性剂共混物的HLB值与所得微凝胶的粒度和催化性能的相关性。
In a proof of concept study, a series of nonionic surfactant blends derived from Tween 80 and Span 80 were used to prepare catalytic microgels from stabilized droplets in miniemulsions. The goal of this study is to optimize the catalytic efficiency of microgels by decreasing their particle size with surfactants that are custom-made for the respective prepolymerization mixture. The effectiveness of the approach is examined by evaluating the catalytic efficiency of the resulting microgels in comparison to their counterparts made in the presence of ionic sodium dodecyl sulfate (SDS) solution. Spherical particles with hydrophilic–lipophilic balance (HLB) value dependent mean hydrodynamic diameters between 99 and 200 nm are obtained. Addition of Cu(II) ions and selected other transition metal ions activated the dormant catalysts for cleavage of glycosidic bonds in 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES) buffer at pH 7.00 and 37 °C using a fluorescent model substrate. The highest proficiency for the catalytic hydrolysis was observed for Cu(II)-containing microgels (kcat/KM × knon = 870 000) with the lowest diameter indicating an almost twofold better stabilization of the transition state compared to a microgel prepared in the presence of ionic SDS solution. The study establishes a correlation of the HLB value of the nonionic surfactant blend used during material synthesis to the particle size and catalytic performance of the resulting microgels.