Effect of nano-hydroxyapatite on bone morphogenetic protein-2-induced hard tissue formation and dentin resorption on a dentin surface
Effect of nano-hydroxyapatite on bone morphogenetic protein-2-induced hard tissue formation and dentin resorption on a dentin surface
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纳米羟基磷灰石对骨形态发生蛋白2诱导的牙本质表面硬组织形成和牙本质吸收的影响
DOI：
10.1016/j.apsusc.2012.03.103
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发表时间：
2012
影响因子：
6.7
通讯作者：
Kawanami M
中科院分区：
文献类型：
--
作者：
Tamagawa H;Tenkumo T;Sugaya T;Kawanami M
目的研究纳米羟基磷灰石加入重组人骨形态发生蛋白-2 （rhBMP-2）胶原膜载体对牙本质表面硬组织形成和牙本质吸收的影响。材料与方法将纳米羟基磷灰石胶原复合膜（nHAC）或胶原膜(C)分别浸入100或400 μg/ml rhBMP-2中，置于植入大鼠大腿肌的牙本质芯片上。分别于术后2周、4周对种植体进行组织学观察和组织形态学分析。结果除400 μg/ml rhBMP-2组在植入后4周形成硬组织外，各nHAC组形成硬组织的比例均显著高于其他C组。nHAC组和C组牙本质吸收百分比在任何阶段和任何rhBMP-2浓度下均无显著差异。结论低剂量rhBMP-2在大鼠大腿肌内植入后早期，在胶原膜上添加纳米羟基磷灰石可促进牙本质表面硬组织的形成，但不增加牙本质的吸收。
AimThe purpose of this study was to evaluate the effects of the addition of nano-hydroxyapatite to a collagen membrane-carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) on hard tissue formation and dentin resorption on dentin surfacesin vivo.Materials and methodsNano-hydroxyapatite collagen composite (nHAC) membranes or collagen (C) membranes were each immersed in either 100 or 400 μg/ml rhBMP-2 and placed on dentin chips that were implanted into rat thigh muscle. The implants were analyzed at 2 or 4 weeks after surgery by histological observation and histomorphometric analysis.ResultsThe percentage of the hard tissue formed by each nHAC group was significantly higher than that formed by any of the C groups, except for that formed by the group loaded with 400 μg/ml rhBMP-2 at 4 weeks after implantation. No significant differences were observed in the percentage of dentin resorption between the nHAC groups and C groups at any stage or at any rhBMP-2 concentration.ConclusionThese findings showed that addition of nano-hydroxyapatite to a collagen membrane accelerated the formation of hard tissue induced by a low dose of rhBMP-2 on dentin surfaces at an early stage after implantation into rat thigh muscle, without increasing dentin resorption.