Analysis of a lectin microarray identifies altered sialylation of mouse serum glycoproteins induced by whole-body radiation exposure
Analysis of a lectin microarray identifies altered sialylation of mouse serum glycoproteins induced by whole-body radiation exposure
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DOI：
10.1093/jrr/rry100
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发表时间：
2019-03-01
影响因子：
2
通讯作者：
Kamiya, Kenji
中科院分区：
文献类型：
--
作者：
Iizuka, Daisuke;Izumi, Shunsuke;Kamiya, Kenji
分析了含有45种不同凝集素的微阵列，以确定暴露于全身γ辐射的小鼠血清糖蛋白糖基化的总体变化。结果表明，辐射暴露分别增加和减少α-2，3-和α-2，6-唾液酸的相对量。分析肝脏中α-2，3-和α-2，6-唾液酸转移酶基因的表达，以确定其表达的变化是否是唾液酸变化的原因。α-2，3-唾液酸的增加与辐射暴露后St 3gal 5的上调相关;然而，未观察到St 6 gal 1表达的降低。对辐照小鼠肝脏中表达的基因进行PCR阵列分析发现，辐照并没有改变大多数基因的表达。这些结果表明，糖组学筛选血清糖蛋白凝集素微阵列可以是一个强大的工具，用于识别辐射诱导的变化，在翻译后添加糖部分的蛋白质。此外，结果表明，改变唾液酸化的糖蛋白可能是一个初始的反应，急性辐射暴露。
Microarrays containing 45 different lectins were analyzed to identify global changes in the glycosylation of serum glycoproteins from mice exposed to whole-body gamma-radiation. The results showed that radiation exposure increased and decreased the relative amounts of alpha-2,3- and alpha-2,6-sialic acids, respectively. The expression of alpha-2,3- and alpha-2,6-sialyltransferase genes in the liver was analyzed to determine whether changes in their expression were responsible for the sialic acid changes. The increase in alpha-2,3-sialic acid correlated with St3gal5 upregulation after radiation exposure; however, a decrease in St6gal1 expression was not observed. Analysis of a PCR array of genes expressed in irradiated mouse livers revealed that irradiation did not alter the expression of most of the included genes. These results suggest that glycomic screening of serum glycoproteins using lectin microarrays can be a powerful tool for identifying radiation-induced changes in the post-translational addition of sugar moieties to proteins. In addition, the results indicate that altered sialylation of glycoproteins may be an initial response to acute radiation exposure.