Polyethylene Glycol-Engrafted Graphene Oxide as Biocompatible Materials for Peptide Nucleic Acid Delivery into Cells
Polyethylene Glycol-Engrafted Graphene Oxide as Biocompatible Materials for Peptide Nucleic Acid Delivery into Cells
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DOI：
10.1021/acs.bioconjchem.8b00025
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发表时间：
2018-02-01
影响因子：
4.7
通讯作者：
Kim, Dong-Eun
中科院分区：
文献类型：
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作者：
Baek, Ahruem;Baek, Yu Mi;Kim, Dong-Eun
已知氧化石墨烯（GO）强烈结合单链核酸，其中在GO表面附近荧光淬灭。然而，GO表现出弱的生物相容性特征，例如在细胞培养基中的低溶解度和显著的细胞毒性。为了提高GO在细胞培养基中的稳定性和细胞活力，我们制备了纳米GO（nGO）构建体并使用聚乙二醇（PEG-nGO）对nGO表面进行修饰。单链肽核酸（PNA）被吸附到PEG-nGO上，并且通过添加互补RNA或在低pH条件下容易地解吸。吸附在PEG-nGO上的PNA通过内吞作用有效地递送到肺癌细胞中，而不影响细胞活力。此外，使用PEG-nGO递送的反义PNA有效地下调癌细胞中靶基因的表达。我们的研究结果表明，PEG-nGO是一种生物相容性载体，可用于将PNA递送到细胞中，并作为一种有前途的基因递送工具。
Graphene oxide (GO) is known to strongly bind single-stranded nucleic acids with fluorescence quenching near the GO surface. However, GO exhibits weak biocompatibility characteristics, such as low dispersibility in cell culture media and significant cytotoxicity. To improve dispersibility in cell culture media and cell viability of GO, we prepared nanosized GO (nGO) constructs and modified the nGO surface using polyethylene glycol (PEG-nGO). Single-stranded peptide nucleic acid (PNA) was adsorbed onto the PEG-nGO and was readily desorbed by adding complementary RNA or under low pH conditions. PNA adsorbed on the PEG-nGO was efficiently delivered into lung cancer cells via endocytosis without affecting cell viability. Furthermore, anti sense PNA delivered using PEG-nGO effectively downregulated the expression of the target gene in cancer cells. Our results suggest that PEG-nGO is a biocompatible carrier useful for PNA delivery into cells and serves as a promising gene delivery tool.