Combination of ultrasound and bubble liposome enhance the effect of doxorubicin and inhibit murine osteosarcoma growth
Combination of ultrasound and bubble liposome enhance the effect of doxorubicin and inhibit murine osteosarcoma growth
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DOI：
10.4161/cbt.12.4.16259
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发表时间：
2011-08-15
影响因子：
3.6
通讯作者：
Komiya, Setsuro
中科院分区：
文献类型：
--
作者：
Ueno, Yoshinori;Sonoda, Shozo;Komiya, Setsuro
如果对细胞施加超声波（US），细胞膜的渗透性会暂时增加，使药物更容易从细胞膜周围进入细胞。此外，当与Bubble脂质体联合使用时，（BL：在本研究中，我们构建了一种新的药物递送系统（DDS），该系统包括US和BL与多柔比星（DOX）的联合使用，多柔比星是骨肉瘤化疗的关键药物，并在体外和体内证明其显著抑制骨肉瘤细胞的增殖。此外，该系统在约1/5剂量的DOX单一疗法中使用的抗肿瘤剂下实现了等效的抗肿瘤效果。这些发现表明了减少不良事件的可能性。在本实验中，我们测试了US和脂质体，两者都已在临床实践中使用。US和脂质体在体内都是非常安全的。我们设计的这些元素组成的DDS可以应用在简单的和特定部位的方式，因此有希望作为一种新的，临床上可行的治疗方法。
If ultrasound (US) is applied to cells, permeability across the cell membrane temporarily increases, making it easier for drugs to be taken into the cells from around the cell membrane. Moreover, when used in combination with Bubble liposome (BL: liposomes which entrap an ultrasound imaging gas), even low-power ultrasound can facilitate drug delivery into cells.In the present study, we constructed a new drug delivery system (DDS) involving concomitant use of US and BL with doxorubicin (DOX), a key drug in the chemotherapy of osteosarcoma, and demonstrated both in vitro and in vivo that it markedly inhibited the proliferation of osteosarcoma cells. Furthermore, this system achieved an equivalent antitumor effect at about 1/5 the dose of antitumor agent employed in monotherapy with DOX. These findings suggest the possibility of reduction of adverse events.In this experiment, US and liposomes were tested, both of which are already in use in clinical practice. US and liposomes are both very safe in the body. The DDS composed of these elements we designed can be applied in simple and site-specific fashion and is therefore promising as a new, clinically feasible method of treatment.