Preparation of Connexin43-Integrated Giant Liposomes by a Baculovirus Expression Liposome Fusion Method
Preparation of Connexin43-Integrated Giant Liposomes by a Baculovirus Expression Liposome Fusion Method
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DOI：
10.1002/bit.22845
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发表时间：
2010-12-01
影响因子：
3.8
通讯作者：
Akiyoshi, Kazunari
中科院分区：
文献类型：
--
作者：
Kamiya, Koki;Tsumoto, Kanta;Akiyoshi, Kazunari
用杆状病毒表达法制备含连接蛋白43(Cx43)的巨型脂质体(GL)。方法制备表达Cx43的重组芽状病毒，与含DOPG/DOPC的GLS在pH=4条件下融合，在透射电子显微镜下观察到连接蛋白在GL膜上的形成，观察到Cx43介导的亲水性荧光染料传递途径，不仅在Sf9细胞与Cx43之间，而且在表达Cx43的U2OS细胞(人骨肉瘤细胞)之间也有亲水性荧光染料转移。脂质体融合方法将创造新的机会作为分析通道膜蛋白Biotechnol BioEng 2010,107 836-843(C)2010 Wiley Periodicail的工具，Inc.
Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression liposome fusion method Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4 5 Connexon formation on the GL membrane was observed by transmission electron microscope Hydrophilic fluorescent dye transfers were observed through a Cx43-mediated pathway not only between Sf9 (Spodoptera frugiperda) cells with Cx43 but also from giant Cx43 liposomes to Cx43-expressing U2OS cells (human osteosarcoma cell) The functional connexin-containing liposome is expected to be useful for cellular cytosolic delivery systems The original orientation and function of Cx43 was maintained after integration into the liposomes The liposome fusion method will create new opportunities as a tool for analysis of channel membrane proteins Biotechnol Bioeng 2010,107 836-843 (C) 2010 Wiley Periodicails, Inc