Fibronectin promotes epithelial migration of cultured rabbit cornea in situ
Fibronectin promotes epithelial migration of cultured rabbit cornea in situ
复制标题
纤连蛋白促进原位培养兔角膜上皮迁移
DOI：
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发表时间：
1983
影响因子：
7.8
通讯作者：
Kiyoshi Watanabe
中科院分区：
文献类型：
--
作者：
Teruo Nishida;S. Nakagawa;Yuichi Ohashi;Kiyoshi Watanabe
我们研究了纤维连接蛋白对培养的兔角膜上皮向基质迁移的影响。用明胶-Sepharose4B亲和层析法纯化兔血浆纤维连接蛋白，并用SDS-聚丙烯酰胺平板凝胶电泳法鉴定其纯度。用豚鼠制备的抗兔血浆纤维连接蛋白抗体与兔血浆形成一条沉淀线，经Ouchterlony双扩散试验纯化的兔血浆纤维连接蛋白。将兔角膜切成小块，单独在Tcm-199培养液中培养时，角膜上皮细胞开始在角膜基质的切缘迁移。在培养液中加入纯化的兔血浆纤维连接蛋白显著促进上皮细胞迁移。增强的程度取决于纤维连接蛋白的添加量。加入抗兔血浆纤维连接蛋白的豚鼠Ig G后，角膜上皮细胞的迁移明显受到抑制。结果表明，纤维连接蛋白促进角膜上皮细胞的迁移，从而在角膜伤口愈合中发挥重要作用。
We investigated the effect of fibronectin on epithelial migration onto the stroma in cultured rabbit cornea. Rabbit plasma fibronectin was purified by affinity chromatography using gelatin-Sepharose 4B, and its purity was confirmed by SDS polyacrylamide slab gel electrophoresis. Antibody against rabbit plasma fibronectin raised in guinea pigs formed a single precipitin line against rabbit plasma and purified rabbit plasma fibronectin by Ouchterlony double diffusion test. When rabbit cornea was cut into small blocks and cultured in TCM-199 medium alone, corneal epithelial cells began to migrate on the cut edge of the corneal stroma. The addition of purified rabbit plasma fibronectin to the culture medium significantly enhanced epithelial migration. The degree of enhancement depended on the amount of fibronectin added. When guinea pig IgG anti-rabbit plasma fibronectin was added, epithelial migration was significantly inhibited when compared with that in control cultured corneal blocks. The results demonstrate that fibronectin promotes epithelial migration in the cornea and thus plays an important role in corneal wound healing.
纤连蛋白治疗兔角膜伤口。
DOI：
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发表时间：
1981
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Fujikawa,LS;Foster,CS;Harrist,TJ;Lanigan,JM;Colvin,RB
通讯作者：
Colvin,RB
人血浆纤连蛋白片段增强人单核细胞不依赖于调理素的吞噬作用。
DOI：
10.1073/pnas.78.6.3649
发表时间：
1981
影响因子：
11.1
作者：
Czop,JK;Kadish,JL;Austen,KF
通讯作者：
Austen,KF