Lab-on-a-chip for analysis of triglycerides based on a replaceable enzyme carrier using magnetic beads.
Lab-on-a-chip for analysis of triglycerides based on a replaceable enzyme carrier using magnetic beads.
复制标题
用于使用磁珠分析基于可更换酶载体的甘油三酯的芯片实验室。
DOI：
--
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Hongyuan Chen
中科院分区：
文献类型：
--
作者：
Shao;Xiaodong Yu;Jingjuan Xu;Hongyuan Chen
将脂肪酶、甘油酶(GK)和甘油-3-磷酸氧化酶(GPOx)共固定在壳聚糖/Fe(3)O(4)壳-核复合纳米粒子上，结合微流控芯片技术和磁珠的优点，研制了一种以金纳米带微电极为检测电极的酶载体微流控芯片，用于甘油三酯的检测。该方法使得微芯片酶载体在每次测定后能够以高度重复性的方式容易地更新。对工作电位、缓冲液pH、三磷酸腺苷浓度(ATP、mm)、分离电压和温度等操作参数进行了评价和优化。酶载体微流控芯片检测甘油三酯的线性范围为0~4.0 mm，线性范围为0~10.0 mm，检测下限为15μM~6.0μM，酶载体的长度在1.0~3.0 cm之间变化，酶载体放置40天后仍保持70%的活力。该系统已成功应用于血清中甘油三酯的在线检测。实验结果表明，基于磁珠基微流控芯片的酶载体为准确测定血清中甘油三酯提供了一种相对简单、灵敏、微型化、可替代的手段。
In this paper an enzyme-carrier-based microfluidic chip coupled with a gold nanoband microelectrode as electrochemical detector for Triglyceride (TG) determination was developed by co-immobilized lipase, Glycerokinase (GK) and glycerol-3-phosphate oxidase (GPOx) on chitosan/Fe(3)O(4) composite nanoparticles with a shell-core structure, which combined the advantageous features of microfluidic chips technology with magnetic beads. This procedure enabled the easy renewal of the microchip enzyme carrier after each determination in a highly reproducible manner. Several operational parameters such as working potential, buffer pH, adenosine triphosphate concentrations (ATP, mM), separation voltage and temperature were evaluated and optimized. The performance of enzyme-carrier-based microfluidic chip for TG determination was modulated by changing the length of enzyme carrier from 1.0 to 3.0 cm, and the linear ranges were changed from 0-4.0 mM to 0-10.0 mM with the detection limits from 15 μM to 6.0 μM. The enzyme carrier remained its 70% activity after 40 days storage. This system was successfully employed for on-line detection of TG in serums. The experimental results demonstrated that this enzyme carrier using magnetic beads based microfluidic chip provided a relatively simple, sensitive, miniature, and replaceable means for the accurate determination of TG in serum.
影响因子：
7.4
作者：
Cooper, JW;Chen, JZ;Lee, CS
通讯作者：
Lee, CS
影响因子：
7.4
作者：
Peterson, DS;Rohr, T;Fréchet, JMJ
通讯作者：
Fréchet, JMJ