Stability of reference proteins in human placenta: General protein stains are the benchmark
Stability of reference proteins in human placenta: General protein stains are the benchmark
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DOI：
10.1016/j.placenta.2011.12.008
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发表时间：
2012-03-01
期刊：
影响因子：
3.8
通讯作者：
Vaillancourt, C.
中科院分区：
文献类型：
--
作者：
Lanoix, D.;St-Pierre, J.;Vaillancourt, C.
The stability of reference proteins in semi-quantitative Western blot experiments in normal and diseased placenta has never been studied. This study aims to determine the stability of five reference proteins and two general protein stains in placentas from preeclampsia, geStational diabetes mellitus and matched control pregnancies. The stability of the reference proteins was analysed using indicators of inter-group (P value) and intra-group (coefficient of variation) stability. The effect of different normalization strategies was determined by normalizing serotonin transporter (SERT) expression against the different reference protein markers. Results show significant expression variability of beta-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylproly1 isomerase A (PPIA) and alpha-tubulin, and that amido black staining is the most stable reference protein marker. Furthermore, results show that SERT expression significantly differs according to the reference protein markers used for its normalization. The present study demonstrated the importance of using stable reference protein markers and normalization strategy in order to get correct results in semiquantitative Western blot experiments in placental tissues. ' Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
