BRUGIA-PAHANGI - EFFECTS OF MELANIZATION ON THE UPTAKE OF NUTRIENTS BY MICROFILARIAE IN-VITRO
BRUGIA-PAHANGI - EFFECTS OF MELANIZATION ON THE UPTAKE OF NUTRIENTS BY MICROFILARIAE IN-VITRO
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DOI：
10.1006/expr.1995.1094
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发表时间：
1995-08-01
影响因子：
2.1
通讯作者：
CHEN, CS
中科院分区：
文献类型：
--
作者：
CHEN, CC;CHEN, CS
应用离体黑化和放射自显影技术研究了黑化对巴氏丝虫微丝蚴营养吸收的影响。发现体外在微丝蚴周围形成的黑色素囊完全抑制以下放射性标记的营养物质进入微丝蚴组织：D-[6-H-3]葡萄糖，L-[4，5-H-3]亮氨酸，[2-H-3]甘氨酸，[1-C-14]亚油酸，[5，6，8，9，11，12，14，15-H-3]花生四烯酸，[2，8-H-3]腺嘌呤，[8-C-14]鸟嘌呤HCl，和[5，6-H-3]尿嘧啶。这些结果提供了强有力的证据，黑化可能会杀死微丝蚴饥饿。(C)出版社：Academic Press
The effects of melanization on nutrient uptake by the microfilariae of Brugia pahangi were investigated by using in vitro melanization and autoradiographic techniques. It was found that the melanotic capsules which formed around the microfilariae in vitro completely inhibited the uptake of the following radiolabeled nutrients into microfilarial tissues: D-[6-H-3]glucose, L-[4,5-H-3]leucine, [2-H-3]glycine, [1-C-14]linoleic acid, [5,6,8,9,11,12,14,15-H-3]arachidonic acid, [2,8-H-3]adenine, [8-C-14]guanine HCl, and [5,6-H-3]uracil. These results provide strong evidence that melanization may kill the microfilariae by starvation. (C) 1995 Academic Press, Inc.