Antiviral effect of catechins in green tea on influenza virus
Antiviral effect of catechins in green tea on influenza virus
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DOI：
10.1016/j.antiviral.2005.06.010
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发表时间：
2005-11-01
影响因子：
7.6
通讯作者：
Seong, BL
中科院分区：
文献类型：
--
作者：
Song, JM;Lee, KH;Seong, BL
评价了来自绿色茶的多酚化合物儿茶素（（-）-表没食子儿茶素没食子酸酯（EGCG）、（-）-表儿茶素没食子酸酯（ECG）和（-）-表没食子儿茶素（EGC））在细胞培养物中抑制流感病毒复制的能力和潜在的直接杀病毒作用。在测试化合物中，发现EGCG和ECG是MDCK细胞培养物中流感病毒复制的有效抑制剂，并且在所有测试的流感病毒亚型（包括A/H1N1、A/H3 N2和B病毒）中观察到这种效果。EGCG、ECG和EGC对甲型流感病毒的50%有效抑制浓度（EC 50）分别为22-28、22-40和309-318 μ M。EGCG和ECG均具有血凝抑制活性，其中EGCG的作用更强。然而，血凝抑制的灵敏度在所测试的三种不同亚型的流感病毒之间存在很大差异。定量RT-PCR分析显示，在高浓度下，EGCG和ECG也抑制MDCK细胞中的病毒RNA合成，而EGC未能显示类似的效果。类似地，EGCG和ECG比EGC更有效地抑制神经氨酸酶活性。结果表明，儿茶素骨架的3-没食子酰基对所观察到的抗病毒活性起着重要作用，而2-位三羟基苄基部分的5 '-OH起着次要作用。结果，沿着HA类型特异性效应，表明儿茶素对流感病毒的抗病毒作用不仅通过与HA的特异性相互作用介导，而且改变了病毒膜的物理性质。(c)2005 Elsevier B. V.保留所有权利。
Polyphenolic compound catechins ((-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG) and (-)-epigallocatechin (EGC)) from green tea were evaluated for their ability to inhibit influenza virus replication in cell culture and for potentially direct virucidal effect. Among the test compounds, the EGCG and ECG were found to be potent inhibitors of influenza virus replication in MDCK cell culture and this effect was observed in all influenza virus subtypes tested, including A/H1N1, A/H3N2 and B virus. The 50% effective inhibition concentration (EC50) of EGCG, ECG, and EGC for influenza A virus were 22-28, 22-40 and 309-318 mu M, respectively. EGCG and ECG exhibited hemagglutination inhibition activity, EGCG being more effective. However, the sensitivity in hemagglutination inhibition was widely different among three different subtypes of influenza viruses tested. Quantitative RT-PCR analysis revealed that, at high concentration, EGCG and ECG also suppressed viral RNA synthesis in MDCK cells whereas EGC failed to show similar effect. Similarly, EGCG and ECG inhibited the neuraminidase activity more effectively than the EGC. The results show that the 3-galloyl group of catechin skeleton plays an important role on the observed antiviral activity, whereas the 5'-OH at the trihydroxy benzyl moiety at 2-position plays a minor role. The results, along with the HA type-specific effect, suggest that the antiviral effect of catechins on influenza virus is mediated not only by specific interaction with HA, but altering the physical properties of viral membrane. (c) 2005 Elsevier B.V. All rights reserved.