Infrared spectroscopic study of the gel to liquid-crystal phase transition in live Acholeplasma laidlawii cells.
Infrared spectroscopic study of the gel to liquid-crystal phase transition in live Acholeplasma laidlawii cells.
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DOI：
10.1021/bi00337a016
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发表时间：
1985-07
期刊：
影响因子：
2.9
通讯作者：
D. Cameron;A. Martin;D. Moffatt;H. Mantsch
中科院分区：
文献类型：
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作者：
D. Cameron;A. Martin;D. Moffatt;H. Mantsch
氘标记的质膜的活Acholeplasma laidlawii B细胞和分离的质膜的红外光谱的温度依赖性表明，该公司的凝胶到液晶相变是非常不同的。在相变范围内的温度下，活支原体能够将其质膜的“流动性”保持在比相同温度下分离的质膜的流动性高得多的值。这种差异在生长温度及其附近特别明显。活的莱氏无胆甾原体在37 ℃下在补充有肉豆蔻酸（C14：0）、十五烷酸（C15：0）或棕榈酸（C16：0）的脂肪酸耗尽的培养基上生长，是高度“流动的”;即，在生长温度下，在37 ℃下液晶相的分数群体为95-100%，而在分离质膜的情况下，在37 ℃下液晶相的分数群体仅为58%（C14：0）、36%（C15：0）或38%（C16：0）。
The temperature dependences of the infrared spectra of deuterium-labeled plasma membranes of live Acholeplasma laidlawii B cells and of the isolated plasma membranes demonstrate that the profiles of the gel to liquid-crystal phase transitions are very different. At temperatures within the range of the phase transition, the live mycoplasma is able to keep the "fluidity" of its plasma membrane at a much higher value than that of the isolated plasma membrane at the same temperature. The difference is particularly pronounced at and around the temperature of growth. Live Acholeplasma laidlawii, grown at 37 degrees C on a fatty acid depleted medium supplemented with myristic acid (C14:0), pentadecanoic acid (C15:0), or palmitic acid (C16:0), are highly "fluid"; i.e., at the temperature of growth, the fractional population of the liquid-crystalline phase is 95-100% at 37 degrees C, whereas in the case of the isolated plasma membranes the fractional population of the liquid-crystalline phase at 37 degrees C is only 58% (C14:0), 36% (C15:0), or 38% (C16:0).