Simultaneous determination of glycyrrhizin, glycyrrhetic acid and glycyrrhetic acid mono-glucuronide in Shakuyaku-kanzo-to incubated with rat feces by semi-micro high-performance liquid chromatography
Simultaneous determination of glycyrrhizin, glycyrrhetic acid and glycyrrhetic acid mono-glucuronide in Shakuyaku-kanzo-to incubated with rat feces by semi-micro high-performance liquid chromatography
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DOI：
10.1248/bpb.24.1161
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发表时间：
2001-10-01
影响因子：
2
通讯作者：
Yagi, A
中科院分区：
文献类型：
--
作者：
Okamura, N;Maki, T;Yagi, A
建立了半微量高效液相色谱法（HPLC）同时测定大鼠粪便中甘草次酸（GA）、甘草次酸单葡萄糖苷酸（GAMG）和甘草苷（GL）的含量。使用TSKgel ODS-80 TsQA（150 X 2.0 min i.d.）柱，使用含有磷酸水溶液和乙腈的移动的相以0.2 ml的流速进行线性梯度洗脱。min（-1），恒温箱25 ℃，检测波长254 nm。这些化合物的检测限为0.1-0.85 pmol/注射（5穆尔）。与未处理的对照组相比，连续食用Shakuyaku-kanzo-to后孵育混合物中GL及其代谢产物的浓度有显着差异。预先食用Shakuyaku-kanzo-to可增强大鼠粪便的GL-水解。
A method for semi-micro high-performance liquid chromatography (HPLC) has been established for the simultaneous determination of glycyrrhizin (GL), glycyrrhetic acid (GA) and glycyrrhetic acid mono-glucuronide (GAMG) in incubation mixtures of rat feces with Shakuyaku-kanzo-to decoction (combination of licorice root and peony root). The analysis could be accomplished within 20 min with a TSKgel ODS-80TsQA (150 X 2.0 min i.d.) column by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile at a flow rate of 0.2 ml . min(-1), a thermostatic oven at 25 degreesC, and detection at 254 nm. The detection limits of these compounds were 0.1-0.85 pmol per injection (5 mul). The concentrations of GL and its metabolites in the incubation mixture after continuous consumption of Shakuyaku-kanzo-to were significantly different compared with those of untreated control. GL-hydrolysis of rat feces was enhanced by pre-consumption of Shakuyaku-kanzo-to.