Distribution, Translocation and Accumulation of Silver Nanoparticles in Rats
Distribution, Translocation and Accumulation of Silver Nanoparticles in Rats
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纳米银粒子在大鼠体内的分布、易位和积累
DOI：
10.1166/jnn.2009.1269
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发表时间：
2009-08-01
影响因子：
--
通讯作者：
Xi, Tingfei
中科院分区：
文献类型：
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作者：
Tang, Jinglong;Xiong, Ling;Xi, Tingfei
银纳米粒子（SNPs）广泛应用于生物医学领域，但对其在体内的分布及其诱导的毒性仍知之甚少。本研究旨在探讨皮下注射大鼠snp的分布和积累。给大鼠注射62.8 mg/kg的snp或银微粒（SMPs），然后在预定时间点处死。采集实验动物的主要器官，用透射电子显微镜（TEM）进行超微结构分析，用电感耦合等离子体质谱（ICP-MS）进行银含量分析。结果表明，snp转运至血液循环，分布于各主要脏器，以颗粒形式分布于肾、肝、脾、脑和肺。然而，SMPs不能侵入血流或器官组织。超微结构观察表明，这些在器官内积累的snp可以进入不同种类的细胞，如肾小管上皮细胞和肝细胞。此外，SNPs还引起血脑屏障（BBB）破坏和星形胶质细胞肿胀，并引起神经元变性。结果表明，snp在生物医学上的应用需要更多的注意，特别是长期使用。
Silver nanoparticles (SNPs) are widely used in the field of biomedicine, but a comprehensive understanding of how SNPs distribute in the body and the induced toxicity remains largely unknown. The present study was designed to investigate the distribution and accumulation of SNPs in rats with subcutaneous injection. Rats were injected with either SNPs or silver microparticles (SMPs) at 62.8 mg/kg, and then sacrificed at predetermined time points. The main organs of the experimental animals were harvested for ultrastructural analysis by transmission electron microscopy (TEM) and for silver content analysis by inductively coupled plasma mass spectrometry (ICP-MS). Results indicated that SNPs translocated to the blood circulation and distributed throughout the main organs, especially in the kidney, liver, spleen, brain and lung in the form of particles. SMPs, however, could not invade the blood stream, or organ tissues. Ultrastructural observations indicate that those SNPs that had accumulated in organs could enter different kinds of cells, such as renal tubular epithelial cells and hepatic cells. Moreover, SNPs also induced blood-brain barrier (BBB) destruction and astrocyte swelling, and caused neuronal degeneration. The results suggest more cautions needed in biomedical applications of SNPs, in particular, the long term uses.