Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds
Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds
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DOI：
10.3892/ijmm.2012.1199
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发表时间：
2013-02-01
影响因子：
5.4
通讯作者：
Pang, Xining
中科院分区：
文献类型：
--
作者：
Liu, Xiaoyu;Wang, Zhe;Pang, Xining
尽管来自羊膜 (hAMSC)、骨髓 (hBMSC) 和脂肪组织 (hADSC) 的人间充质干细胞 (MSC) 移植已被证明有助于小鼠模型皮肤伤口的修复，但关于不同来源 MSC 的相对功效的信息很少。在这项研究中，我们通过在皮肤伤口小鼠模型中移植相同数量的 hAMSC、hBMSC 或 hADSC 来比较它们的治疗潜力。结果表明，hADSC 注射对伤口闭合具有最显着的效果。组织学评估显示，与 hBMSC 和 hAMSC 组相比，hADSC 组的再上皮化增强。尽管hAMSC和hBMSC组的伤口愈合略有改善，但各组之间的差异在统计学上并不显着。在 Transwell 共培养模型中，伤口愈合迁移和 Transwell 迁移测定表明，hADSC 在促进人真皮成纤维细胞 (hDF) 迁移方面优于 hAMSC 和 hBMSC。然而，通过 WST 测定测量，hAMSC、hBMSC 和 hADSC 组之间的成纤维细胞增殖没有显着差异。我们的结果还表明，hDF与hADSC共培养48小时显着上调细胞因子血管内皮生长因子、碱性成纤维细胞生长因子、角质形成细胞生长因子和转化生长因子13的mRNA表达，并增加I型胶原的mRNA和蛋白水平。总的来说，这些数据表明，就功效、可及性和可用性而言，hADSC 是皮肤伤口治疗性愈合的潜在 MSC 来源。
Although transplantation of human mesenchymal stem cells (MSCs) derived from amnion (hAMSCs), bone marrow (hBMSCs) and adipose tissues (hADSCs) has been shown to aid in the repair of cutaneous wounds in mouse models, little information is available regarding the relative efficacy of MSCs from different sources. In this study, we compared their therapeutic potentials by transplanting equal numbers of hAMSCs, hBMSCs or hADSCs in a mouse model of cutaneous wounds. The results suggested that an hADSC injection has the most pronounced effect on wound closure. Histological evaluation showed enhanced re-epithelialization in the hADSC group compared with the hBMSC and hAMSC groups. Although there was a slight improvement in wound healing in the hAMSC and hBMSC groups, the differences between the groups were statistically insignificant. In a transwell coculture model, wound healing migration and transwell migration assays showed that hADSCs were superior to hAMSCs and hBMSCs at promoting human dermal fibroblast (hDF) migration. However, there was no significant difference in fibroblast proliferation between the hAMSC, hBMSC and hADSC groups, as measured by WST assay. Our results also indicated that hDFs cocultured with hADSCs for 48 h significantly upregulated their mRNA expression of the cytokine vascular endothelial growth factor, basic fibroblast growth factor, keratinocyte growth factor and transforming growth factor-13 and increased the mRNA and protein levels of type I collagen. Collectively, these data suggest that hADSCs are a potential source of MSCs for therapeutic healing in cutaneous wounds in terms of efficacy, accessibility and availability.