Expression of connexin36 in the adult and developing rat brain
Expression of connexin36 in the adult and developing rat brain
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DOI：
10.1016/s0006-8993(00)02300-3
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发表时间：
2000-05-19
期刊：
影响因子：
2.9
通讯作者：
Condorelli, DF
中科院分区：
文献类型：
--
作者：
Belluardo, N;Mudò, G;Condorelli, DF
在蛋白质（免疫荧光）和 mRNA（原位杂交）水平上分析了成年大鼠大脑和视网膜中连接蛋白 36 (Cx36) 的分布。 Cx36 免疫反应性主要由圆形或细长的泪点组成，在特定大脑区域（下橄榄和嗅球）、视网膜、垂体前叶和松果体中高度富集，这与相同区域中高水平的 Cx36 mRNA 一致。在几个大脑区域中可以观察到较低密度的免疫反应性斑点，其中只有分散的细胞亚群表达 Cx36 mRNA。通过将 Cx36 mRNA 原位杂交与一般神经元标记物 (NeuN) 的免疫组织化学相结合，我们发现神经元细胞负责下橄榄、小脑、纹状体、海马和大脑皮层中 Cx36 mRNA 的表达。 Cx36 mRNA 也在大脑皮层、纹状体、海马和小脑皮层的含有小白蛋白的 GABA 能中间神经元中得到证实。对发育中大脑的分析进一步表明，Cx36在出生后的前两周达到表达高峰，并在第三周急剧下降。此外，在出生后发育的早期阶段，在成年阶段缺乏 Cx36 mRNA 的神经元群体中可以检测到 Cx36。 Cx36 表达的发育变化表明该连接蛋白参与了发生在出生后早期大脑的几个区域的广泛的神经元间耦合。 (C) 2000 Elsevier Science B.V. 保留所有权利。
The distribution of connexin36 (Cx36) in the adult rat brain and retina has been analysed at the protein (immunofluorescence) and mRNA (in situ hybridization) level. Cx36 immunoreactivity, consisting primarily of round or elongated puncta, is highly enriched in specific brain regions (inferior olive and the olfactory bulb), in the retina, in the anterior pituitary and in the pineal gland, in agreement with the high levels of Cx36 mRNA in the same regions. A lower density of immunoreactive puncta can be observed in several brain regions, where only scattered subpopulations of cells express Cx36 mRNA. By combining in situ hybridization for Cx36 mRNA with immunohistochemistry for a general neuronal marker (NeuN), we found that neuronal cells are responsible for the expression of Cx36 mRNA in inferior olive, cerebellum, striatum, hippocampus and cerebral cortex. Cx36 mRNA was also demonstrated in parvalbumin-containing GABAergic interneurons of cerebral cortex, striatum, hippocampus and cerebellar cortex. Analysis of developing brain further revealed that Cx36 reaches a peak of expression in the first two weeks of postnatal life, and decreases sharply during the third week. Moreover, in these early stages of postnatal development Cx36 is detectable in neuronal populations that are devoid of Cx36 mRNA at the adult stage. The developmental changes of Cx36 expression suggest a participation of this connexin in the extensive interneuronal coupling which takes place in several regions of the early postnatal brain. (C) 2000 Elsevier Science B.V. All rights reserved.