Identification of the antivasopermeability effect of pigment epithelium-derived factor and its active site
Identification of the antivasopermeability effect of pigment epithelium-derived factor and its active site
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DOI：
10.1073/pnas.0308342101
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发表时间：
2004-04-27
影响因子：
11.1
通讯作者：
Tong, PY
中科院分区：
文献类型：
--
作者：
Liu, H;Ren, JG;Tong, PY
血管通透性在一系列危及生命和视力的疾病中起着关键作用。血管内皮生长因子可增加血管通透性。使用非增殖性糖尿病视网膜病变的模型系统，我们发现色素上皮衍生因子（PEDF）有效地减弱血管内皮生长因子诱导的血管通透性。PEDF的44个氨基酸区域足以赋予抗渗透性活性。此外，我们确定了四种氨基酸（谷氨酸-101，异亮氨酸-103，亮氨酸-112和丝氨酸-115）对这种活性至关重要。PEDF或其衍生物可能减轻或恢复糖尿病黄斑水肿所致的视力丧失。此外，PEDF可能代表败血症相关性低血压、肾病综合征和其他由过度血管通透性引起的危及视力和生命的疾病的上级治疗方法。
Vascular permeability plays a key role in a wide array of life-threatening and sight-threatening diseases. Vascular endothelial growth factor can increase vascular permeability. Using a model system for nonproliferative diabetic retinopathy, we found that pigment epithelium-derived factor (PEDF) effectively abated vascular endothelial growth factor-induced vascular permeability. A 44-amino acid region of PEDF was sufficient to confer the antivasopermeability activity. Additionally, we identified four amino acids (glutamate-101, isoleucine-103, leucine-112, and serine-115) critical for this activity. PEDF, or a derivative, could potentially abate or restore vision loss from diabetic macular edema. Furthermore, PEDF may represent a superior therapeutic approach to sepsis-associated hypotension, nephrotic syndrome, and other sight-threatening and life-threatening diseases resulting from excessive vascular permeability.