Deciphering the diversity of small RNAs in plants: the long and short of it.
Deciphering the diversity of small RNAs in plants: the long and short of it.
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DOI：
10.1093/bfgp/elp024
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
F. Schwach;Simon Moxon;V. Moulton;T. Dalmay
中科院分区：
文献类型：
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作者：
F. Schwach;Simon Moxon;V. Moulton;T. Dalmay
RNA沉默是一种复杂的高度保守的调控机制，目前已知其参与诸如发育、病原体控制、基因组维持和对环境变化的响应等多种过程。RNA沉默自最近被发现以来，已成为动植物分子生物学研究的一个快速发展的关键领域。这一领域的研究极大地受益于新型测序技术的最新发展，这些技术允许对所有RNA沉默现象的关键参与者小RNA（sRNA）分子进行大规模并行测序。随着研究人员开始破译RNA沉默的复杂性，必须开发新的方法来理解目前产生的大量数据。在这篇综述中，我们提出了一个概述的RNA沉默途径在植物和当前的挑战，在分析sRNA数据，特别关注计算方法。
RNA silencing is a complex and highly conserved regulatory mechanism that is now known to be involved in such diverse processes as development, pathogen control, genome maintenance and response to environmental changes. Since its recent discovery, RNA silencing has become a fast moving key area of research in plant and animal molecular biology. Research in this field has greatly profited from recent developments in novel sequencing technologies that allow massive parallel sequencing of small RNA (sRNA) molecules, the key players of all RNA silencing phenomena. As researchers are beginning to decipher the complexity of RNA silencing, novel methodologies have to be developed to make sense of the large amounts of data that are currently being generated. In this review we present an overview of RNA silencing pathways in plants and the current challenges in analysing sRNA data, with a special focus on computational approaches.