STUDIES ON THE MODE OF ACTION OF CALCIFEROL .18. EVIDENCE FOR A SPECIFIC HIGH-AFFINITY BINDING-PROTEIN FOR 1,25 DIHYDROXYVITAMIN-D3 IN CHICK KIDNEY AND PANCREAS
STUDIES ON THE MODE OF ACTION OF CALCIFEROL .18. EVIDENCE FOR A SPECIFIC HIGH-AFFINITY BINDING-PROTEIN FOR 1,25 DIHYDROXYVITAMIN-D3 IN CHICK KIDNEY AND PANCREAS
复制标题
DOI：
10.1016/0006-291x(79)90942-2
复制
发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
NORMAN, AW
中科院分区：
文献类型：
--
作者：
CHRISTAKOS, S;NORMAN, AW
用蔗糖密度梯度离心法分析了鸡肾和胰腺细胞质溶胶中维生素D3代谢物的结合情况。两种细胞质组分均含有一个3.6S大分子，特异结合1,25-二羟基[3H]维生素D3和一个5-6S大分子，特异结合25-羟基[3H]维生素D3。从肾脏或胰腺染色质制备的KCl提取物的蔗糖梯度分析导致结合的1,25-二羟基维生素D3的峰值（3.6S）无法与细胞质结合成分区分。1,25-二羟基[3H]维生素D3与两种组织的细胞质结合成分的相互作用发生在低浓度的高亲和力激素下。
Cytosol fractions prepared from rachitic chick kidney and pancreas were analyzed for binding of vitamin D3 metabolites by sucrose density gradient centrifugation. Both cytosol fractions contained a 3.6S macromolecule which specifically binds 1,25-dihydroxy[3H] vitamin D3 and a 5-6S macromolecule which binds 25-hydroxy[3H]vitamin D3. Sucrose gradient analysis of a KCl extract prepared from kidney or pancreas chromatin resulted in a peak (3.6S) of bound 1,25-dihydroxyvitamin D3 which could not be distinguished from the cytoplasmic binding component. The interaction of 1,25-dihydroxy[3H]vitamin D3 with the cytoplasmic binding component of both tissues occurred at low concentrations of hormone with high affinity.