3D Bioprinting of Vessel-like Structures with Multilevel Fluidic Channels
3D Bioprinting of Vessel-like Structures with Multilevel Fluidic Channels
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DOI：
10.1021/acsbiomaterials.6b00643
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发表时间：
2017-03-01
影响因子：
5.8
通讯作者：
He, Yong
中科院分区：
文献类型：
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作者：
Gao, Qing;Liu, Zhenjie;He, Yong
本研究通过挤压三维生物打印技术，构建了具有多级流体通道(机械刺激的宏通道和营养输送和化学刺激的微通道)的三维水凝胶血管结构，并将其集成到能够更好地模拟血管微环境的芯片上器官装置中。在这种方法中，负载成纤维细胞和平滑肌细胞的部分交联型中空海藻酸丝通过同轴喷嘴挤出，然后沿着旋转的棒状模板打印，然后将内皮细胞种植到内壁。由于相邻的中空纤维的融合，形成了两级流道，包括中间由圆柱形模板形成的宏观通道和由中空纤维形成的围绕壁面的微通道。通过这种方法，打印出了不同形状的毫米直径的血管状结构。用4%海藻酸盐打印的结构显示出0.184 Mpa的极限强度，包裹在结构中的L929小鼠成纤维细胞1周内存活率超过90%。作为概念验证，演示了一种设想的机械和化学刺激的加载系统。此外，还制作了血管循环系统、脑动脉手术模拟器和细胞共培养模型，以展示这些打印结构的潜在组织工程应用。
In this study, 3D hydrogel-based vascular structures with multilevel fluidic channels (macro-channel for mechanical stimulation and microchannel for nutrient delivery and chemical stimulation) were fabricated by extrusion-based three-dimensional (3D) bioprinting, which could be integrated into organ-on-chip devices that would better simulate the microenvironment of blood vessels. In this approach, partially cross-linked hollow alginate filaments loading fibroblasts and smooth muscle cells were extruded through a coaxial nozzle and then printed along a rotated rod template, and endothelial cells were seeded into the inner wall. Because of the fusion of adjacent hollow filaments, two-level fluidic channels, including a macro-channel in the middle formed from the cylindrical template and a microchannel around the wall resulted from the hollow filaments were formed. By this method, different shapes of vessellike structures of millimeter diameter were printed. The structures printed using 4% alginate exhibited ultimate strength of 0.184 MPa, and L929 mouse fibroblasts encapsulated in the structures showed over 90% survival within 1 week. As a proof of concept, an envisioned load system of both mechanical and chemical stimulation was demonstrated. In addition, a vascular circulation flow system, a cerebral artery surgery simulator, and a cell coculture model were fabricated to demonstrate potential tissue engineering applications of these printed structures.