Alkaloids from Corydalis decumbens modulate neuronal excitability
Alkaloids from Corydalis decumbens modulate neuronal excitability
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延胡索中的生物碱调节神经元兴奋性
DOI：
10.1016/j.bioorg.2020.103795
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发表时间：
2020-06-01
影响因子：
5.1
通讯作者：
Cao, Zheng-Yu
中科院分区：
文献类型：
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作者：
Zhang, Chun-Lei;Huang, Qi-Long;Cao, Zheng-Yu
从降香球茎中分得8个新生物碱，包括5个异喹啉生物碱、1个苯并氮杂卓生物碱、2个异吲哚生物碱和3个首次从天然来源得到的合成生物碱，以及3个已知生物碱。通过光谱数据和单晶X-射线衍射分析确定了其结构。本文首次报道了异吲哚类生物碱和苯并氮杂类生物碱。本文首次报道了9-11的完整NMR数据。此外，在原代培养的新皮层神经元中测试了分离的生物碱调节神经元Ca 2+动员的能力。化合物7在低微摩尔浓度下抑制原代新皮层神经元培养物中的自发Ca 2+振荡。
Eight new alkaloids, including five isoquinoline alkaloids, a benzoazepine alkaloid, two isoindole alkaloids, and three synthetic alkaloids firstly obtained from the natural sources, together with three known ones were isolated from the bulbs of Corydalis decumbens. The structures were determined by analysis of their spectroscopic data and single-crystal X-ray diffraction. This is the first report of isoindole alkaloid and benzoazepine alkaloid from the genus Corydalis. Full NMR data for 9-11 are reported here for the first time. Moreover, the ability to modulate neuronal Ca2+ mobilization of the isolated alkaloids was tested in primary cultured neocortical neurons. Compound 7 inhibited spontaneous Ca2+ oscillations in primary neocortical neuron cultures at low micromolar concentrations.