Murine Genetic Background Has a Stronger Impact on the Composition of the Gut Microbiota than Maternal Inoculation or Exposure to Unlike Exogenous Microbiota
Murine Genetic Background Has a Stronger Impact on the Composition of the Gut Microbiota than Maternal Inoculation or Exposure to Unlike Exogenous Microbiota
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DOI：
10.1128/aem.00826-19
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发表时间：
2019-09-01
影响因子：
4.4
通讯作者：
Kashi, Yechezkel
中科院分区：
文献类型：
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作者：
Korach-Rechtman, Hila;Freilich, Shay;Kashi, Yechezkel
肠道微生物群是一个复杂的生态系统，受环境因素和宿主遗传因素的影响。在这里，我们的目的是揭示细菌类群的肠道持久性是由宿主遗传变异控制。我们使用了基于近交系BALB/c和C57 BL/6 J及其F-1正反交杂种（雌性C57 BL/6 J x雄性BALB/c;雌性BALB/c x雄性C57 BL/6 J）的小鼠模型。为了保证F-1后代的遗传相似性，包括性染色体，我们只使用雌性小鼠。基于16 S rRNA基因测序，我们发现遗传上不同的近交系呈现不同的微生物群，而它们遗传上相同的F-1正反交杂种呈现相似的微生物群。此外，F-1的微生物组成不同于两个亲本系。12个分类群被证明具有遗传控制的肠道持久性，而没有被发现表现出母体效应。其中9个类群由C57 BL/6 J系显性遗传。亲本近交系的共饲养导致微生物群组成的暂时和微小的变化，其在分离后返回到先前的微生物组成，这表明每个系倾向于保持反映该系的独特细菌特征。总之，我们的研究结果表明，小鼠遗传学对肠道中的微生物组成有影响，这大于母体效应和持续暴露于替代品系的不同微生物群。揭示与宿主遗传学相关的细菌分类群，并了解它们在肠道生态系统中的作用，可能会导致开发基因导向的益生菌产品，作为个性化医疗方法的一部分。重要信息肠道微生物群对宿主起着重要作用。宿主遗传学与其微生物组成之间的联系越来越受到关注。使用一个独特的互惠交叉模型，产生遗传相似的F-1杂种与不同的母亲接种，我们证明了12个细菌类群的肠道持久性的遗传。没有确定为母系传播的分类群。此外，两种遗传上不同的近交系的同居并没有显着影响微生物群组成。总之，我们的研究结果证明了遗传效应对母体接种或暴露于不同外源性微生物群的影响的重要性。这些发现可能会导致开发个性化的益生菌产品，特别是根据基因组成设计的产品。
The gut microbiota is a complex ecosystem, affected by both environmental factors and host genetics. Here, we aim at uncovering the bacterial taxa whose gut persistence is controlled by host genetic variation. We used a murine model based on inbred lines BALB/c and C57BL/6J and their F-1 reciprocal hybrids (female C57BL/6J x male BALB/c; female BALB/c x male C57BL/6J). To guarantee genetic similarity of F-1 offspring, including the sex chromosomes, we used only female mice. Based on 16S rRNA gene sequencing, we found that the genetically different inbred lines present different microbiota, whereas their genetically identical F-1 reciprocal hybrids presented similar microbiota. Moreover, the F-1 microbial composition differed from that of both parental lines. Twelve taxa were shown to have genetically controlled gut persistence, while none were found to show maternal effects. Nine of these taxa were dominantly inherited by the C57BL/6J line. Cohousing of the parental inbred lines resulted in a temporary and minor shift in microbiota composition, which returned back to the former microbial composition following separation, indicating that each line tends to maintain a unique bacterial signature reflecting the line. Taken together, our findings indicate that mouse genetics has an effect on the microbial composition in the gut, which is greater than maternal effect and continuous exposure to different microbiota of the alternative line. Uncovering the bacterial taxa associated with host genetics and understanding their role in the gut ecosystem could lead to the development of genetically oriented probiotic products, as part of the personalized medicine approach.IMPORTANCE The gut microbiota play important roles for their host. The link between host genetics and their microbial composition has received increasing interest. Using a unique reciprocal cross model, generating genetically similar F-1 hybrids with different maternal inoculation, we demonstrate the inheritance of gut persistence of 12 bacterial taxa. No taxa identified as maternally transmitted. Moreover, cohabitation of two genetically different inbred lines did not dramatically affect the microbiota composition. Taken together, our results demonstrate the importance of the genetic effect over maternal inoculation or effect of exposure to unlike exogenous microbiota. These findings may lead to the development of personalized probiotic products, specifically designed according to the genetic makeup.