Nitric oxide plays a central role in determining lateral root development in tomato
Nitric oxide plays a central role in determining lateral root development in tomato
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DOI：
10.1007/s00425-003-1172-7
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发表时间：
2004-04-01
期刊：
影响因子：
4.3
通讯作者：
Lamattina, L
中科院分区：
文献类型：
--
作者：
Correa-Aragunde, N;Graziano, M;Lamattina, L
一氧化氮(NO)是一种生物活性分子，在植物体内的许多生理过程中发挥作用，其中大部分与传统的植物激素有串扰作用。生长素是调节根系构型的主要激素。在本文中，我们报道了NO促进侧根(LR)的发育，这是一个生长素依赖的过程。NO供体硝普钠(SNP)在番茄上的应用幼苗以剂量依赖的方式诱导主根的萌发和伸长，而主根(PR)的生长受到抑制。这种作用是NO专一性的，因为NO清除剂2-(4-carboxyphenyl)-4，4，5，5-tetramethylimidazoline-1-oxyl-3-oxide阻断了硝普钠的作用。去除内源NO后，根长增加了40%，证明了NO在调控根系生长发育中的生理作用。4，5-二氨基二乙酸二氨基荧光素(DAF-2 DA)对内源性NO的检测表明，在LR原基发育的各个阶段，NO信号都是特异定位的。在另一组实验中，SNP能够促进生长素运输抑制剂N-1-萘基邻苯二甲酸(NPA)处理的生长素枯竭幼苗的LR发育。此外，研究还发现，CPTiO可剂量依赖性地抑制由合成生长素1-萘乙酸(NAA)诱导的LR的形成。综上所述，这些结果表明，NO在LR的发育调节中发挥了新的作用，可能是在生长素信号转导途径中发挥作用。
Nitric oxide (NO) is a bioactive molecule that functions in numerous physiological processes in plants, most of them involving cross-talk with traditional phytohormones. Auxin is the main hormone that regulates root system architecture. In this communication we report that NO promotes lateral root (LR) development, an auxin-dependent process. Application of the NO donor sodium nitroprusside (SNP) to tomato (Lycopersicon esculentum Mill.) seedlings induced LR emergence and elongation in a dose-dependent manner, while primary root (PR) growth was diminished. The effect is specific for NO since the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CPTIO) blocked the action of SNP. Depletion of endogenous NO with CPTIO resulted in the complete abolition of LR emergence and a 40% increase in PR length, confirming a physiological role for NO in the regulation of root system growth and development. Detection of endogenous NO by the specific probe 4,5-diaminofluorescein diacetate (DAF-2 DA) revealed that the NO signal was specifically located in LR primordia during all stages of their development. In another set of experiments, SNP was able to promote LR development in auxin-depleted seedlings treated with the auxin transport inhibitor N-1-naphthylphthalamic acid (NPA). Moreover, it was found that LR formation induced by the synthetic auxin 1-naphthylacetic acid (NAA) was prevented by CPTIO in a dose-dependent manner. All together, these results suggest a novel role for NO in the regulation of LR development, probably operating in the auxin signaling transduction pathway.