Simple replication methods for producing nanoslits in thermoplastics and the transport dynamics of double-stranded DNA through these slits
Simple replication methods for producing nanoslits in thermoplastics and the transport dynamics of double-stranded DNA through these slits
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DOI：
10.1039/c0lc00096e
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Soper, Steven A.
中科院分区：
文献类型：
--
作者：
Chantiwas, Rattikan;Hupert, Mateusz L.;Soper, Steven A.
使用简单而可靠的方法在热塑性塑料中制造混合尺度的纳米和微流体网络，不需要使用复杂的设备来生产纳米结构。高精度微铣削 (HPMM) 和光刻技术用于生成混合规模的成型工具，随后用于将流体网络生产到热塑性塑料中，例如聚甲基丙烯酸甲酯、PMMA、环烯烃共聚物、COC 和聚碳酸酯、PC。使用纳米压印工具将纳米狭缝阵列压印到聚合物中，该工具由光学掩模组成，其图案宽度为 2-7 μm，深度由沉积在玻璃上的 Cr 层 (100 nm) 限定。该器件还包含一个微通道网络，使用 HPMM 制备的金属成型工具将其热压印到聚合物基底中。该混合尺度设备还可用作生产聚合物印模的母版，该聚合物印模由聚二甲基硅氧烷、PDMS 制成，并用于一步生成混合尺度流体网络。通过对基板和盖板进行氧等离子体处理，在低于各自 T-g 的温度下实现盖板与基板的热熔结合，这显着减少了组装过程中热引起的结构变形：与 PMMA 相似 6%，与 COC 纳米狭缝相似 9%。研究了双链 DNA (dsDNA) 通过聚合物纳米狭缝（PMMA 和 COC）的动电传输特性。在这些聚合物器件中，dsDNA 表现出场依赖性电泳迁移率和间歇性传输动力学。在场强为 25 V cm(-1) 时，PMMA 和 COC 的 DNA 迁移率分别为 8.2 +/- 0.7 x 10(-4) cm(2) V-1 s(-1) 和 7.6 +/- 0.6 x 10(-4) cm(2) V-1 s(-1)。纳米狭缝的 lambda-DNA 的延伸因子在 PMMA 中为 0.46，在 COC 中为 0.53（2-6% 标准偏差）。
Mixed-scale nano-and microfluidic networks were fabricated in thermoplastics using simple and robust methods that did not require the use of sophisticated equipment to produce the nanostructures. High-precision micromilling (HPMM) and photolithography were used to generate mixed-scale molding tools that were subsequently used for producing fluidic networks into thermoplastics such as poly(methyl methacrylate), PMMA, cyclic olefin copolymer, COC, and polycarbonate, PC. Nanoslit arrays were imprinted into the polymer using a nanoimprinting tool, which was composed of an optical mask with patterns that were 2-7 mu m in width and a depth defined by the Cr layer (100 nm), which was deposited onto glass. The device also contained a microchannel network that was hot embossed into the polymer substrate using a metal molding tool prepared via HPMM. The mixed-scale device could also be used as a master to produce a polymer stamp, which was made from polydimethylsiloxane, PDMS, and used to generate the mixed-scale fluidic network in a single step. Thermal fusion bonding of the cover plate to the substrate at a temperature below their respective T-g was accomplished by oxygen plasma treatment of both the substrate and cover plate, which significantly reduced thermally induced structural deformation during assembly: similar to 6% for PMMA and similar to 9% for COC nanoslits. The electrokinetic transport properties of double-stranded DNA (dsDNA) through the polymeric nanoslits (PMMA and COC) were carried out. In these polymer devices, the dsDNA demonstrated a field-dependent electrophoretic mobility with intermittent transport dynamics. DNA mobilities were found to be 8.2 +/- 0.7 x 10(-4) cm(2) V-1 s(-1) and 7.6 +/- 0.6 x 10(-4) cm(2) V-1 s(-1) for PMMA and COC, respectively, at a field strength of 25 V cm(-1). The extension factors for lambda-DNA were 0.46 in PMMA and 0.53 in COC for the nanoslits (2-6% standard deviation).