RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics.
RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics.
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肿瘤教育的血小板的RNA-seq可实现基于血液的泛症，多类和分子途径癌症诊断。
DOI：
10.1016/j.ccell.2015.09.018
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发表时间：
2015-11-09
期刊：
影响因子：
50.3
通讯作者：
Wurdinger T
中科院分区：
文献类型：
--
作者：
Best MG;Sol N;Kooi I;Tannous J;Westerman BA;Rustenburg F;Schellen P;Verschueren H;Post E;Koster J;Ylstra B;Ameziane N;Dorsman J;Smit EF;Verheul HM;Noske DP;Reijneveld JC;Nilsson RJA;Tannous BA;Wesseling P;Wurdinger T
受肿瘤训练的血小板（TEP）在对肿瘤生长的全身和局部反应中扮演着重要角色，从而改变了它们的RNA谱。我们通过对283个血小板样本进行mRNA测序来确定TEPs的诊断潜力。我们区分了228例局部和转移性肿瘤患者与55名健康人，准确率为96%。在六种不同的肿瘤类型中，原发性肿瘤的位置被正确识别，准确率为71%。此外，MET或HER2阳性和突变型KRAS、EGFR或PIK3CA肿瘤使用替代TEP mRNA谱准确区分。我们的研究结果表明，血小板为泛癌症、多类癌症和伴随诊断提供了一个有价值的平台，可能使基于血液的“液体活检”的临床进展成为可能。肿瘤通过改变血小板RNA谱“教育”血小板（TEP）TEP为泛癌症、多类和伴随诊断提供RNA生物源基于TEP的液体活检可以指导临床诊断和治疗选择。研究表明，对受肿瘤影响的血小板进行mRNA测序，以96%的准确率将癌症患者与健康个体区分开来，以71%的准确率区分患者的六种原发性肿瘤类型，并鉴定了肿瘤中发现的几种遗传变异。
Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy. Across six different tumor types, the location of the primary tumor was correctly identified with 71% accuracy. Also, MET or HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately distinguished using surrogate TEP mRNA profiles. Our results indicate that blood platelets provide a valuable platform for pan-cancer, multiclass cancer, and companion diagnostics, possibly enabling clinical advances in blood-based “liquid biopsies”. Tumors “educate” platelets (TEPs) by altering the platelet RNA profile TEPs provide a RNA biosource for pan-cancer, multiclass, and companion diagnostics TEP-based liquid biopsies may guide clinical diagnostics and therapy selection A total of 100–500 pg of total platelet RNA is sufficient for TEP-based diagnostics Best et al. show that mRNA sequencing of tumor-educated blood platelets distinguishes cancer patients from healthy individuals with 96% accuracy, differentiates between six primary tumor types of patients with 71% accuracy, and identifies several genetic alterations found in tumors.